Study Cytotoxicity and Genotoxicity of Hemolysin Using Human Lymphocyte | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 7, Volume 14, Issue 2, December 2022, Page 89-98 PDF (547.5 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2022.260027 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Aqeel Hazim Salieh; Batol Imran Dheeb; Asmaa Easa Mahmood | ||||
| Department of pathological analysis, college of applaied science, Samaraa university | ||||
| Abstract | ||||
| The cytotoxic effects on human lymphocytes of Hemolysin sample (one pure sample produced in the laboratory for this study) were assessed at four different concentrations (25, 50, 100 and 200 μL) using the methylthiazol tetrazolium (MTT) bioassay. The results showed that growth was inhibited by 21.4, 32.3, 48.7, and 79.34 for each of the four concentrations of the pure sample, respectively, Deoxyribonucleic acid (DNA) was extracted from the lymphocytes and analysed by electrophoresis on a 1% agarose gel. Hemolysin appeared to have the ability to degrade or damage the DNA. The present study showed that both the growth inhibition and DNA damage experienced by the human lymphocytes increased linearly with increasing concentrations of the toxin. | ||||
| Keywords | ||||
| Cytotoxicity; Genotoxicity; human lymphocytes; Hemolysin | ||||
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