Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection
Ali, Y., Thabet, M., El-Maghraby, A., Gomaa, A., Khamiss, O., Talaat, R. (2022). Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection. EKB Journal Management System, 8(3), 221-235. doi: 10.21608/jbaar.2022.260856
Yasser B.M. Ali; Mohamed G. Thabet; Abeer M. El-Maghraby; Asmaa Gomaa; Omaima A. Khamiss; Roba M. Talaat. "Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection". EKB Journal Management System, 8, 3, 2022, 221-235. doi: 10.21608/jbaar.2022.260856
Ali, Y., Thabet, M., El-Maghraby, A., Gomaa, A., Khamiss, O., Talaat, R. (2022). 'Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection', EKB Journal Management System, 8(3), pp. 221-235. doi: 10.21608/jbaar.2022.260856
Ali, Y., Thabet, M., El-Maghraby, A., Gomaa, A., Khamiss, O., Talaat, R. Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection. EKB Journal Management System, 2022; 8(3): 221-235. doi: 10.21608/jbaar.2022.260856

Interference between miR-21/PTEN/E-Cadherin and Epithelial-Mesenchymal Transition in Various Stages of Chronic HCV Infection

Journal of Bioscience and Applied Research
Volume 8, Issue 3, September 2022, Page 221-235  XML PDF (1.09 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2022.260856
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Authors
Yasser B.M. Aliorcid 1; Mohamed G. Thabet2; Abeer M. El-Maghrabyorcid 2; Asmaa Gomaaorcid 3; Omaima A. Khamissorcid 4; Roba M. Talaat email orcid 2
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
2Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt
3Hepatology and Gastroenterology Department, National Live Institute (NLI), Menufyia University, Shebeen-Elkom, Egypt
4Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt
Abstract
Hepatocellular carcinoma (HCC) is a major complication associated with hepatitis C viral infection (HCV). The epithelial-mesenchymal transition (EMT) is critical in HCC invasion and metastasis. Several microRNAs (miRNAs) have been linked to HCV-related HCC. This study aimed to evaluate the relation between miR-21, phosphatase, tensin homolog deleted on chromosome ten (PTEN), and E-Cadherin with a flashlight on their role in the EMT process in HCV infection at different stages. One hundred HCV-infected patients were studied, 75 had HCV-induced cirrhosis (classified into Child A, B, and C), and 25 had HCC. In parallel, 45 healthy volunteers were considered normal controls. Circulating miR-21was detected by quantitative Reverse Transcription Polymerase Chain Reaction (qRT–PCR).PTEN and E-cadherin serum levels were measured using Enzyme-Linked Immunosorbent Assay (ELISA). A significant elevation in miR-21 was observed in HCC patients compared with control ones(P˂0.01). HCC patients had the lowest E-cadherins and PTEN (P<0.01) compared with cirrhotic and normal subjects. In HCC patients, PTEN was positively correlated with E-cadherin (r= 0.501; p<0.01).  On the other hand, a negative correlation between miR-21 and both E-cadherins (r= -0.455; p<0.01) and PTEN (r= -0.255; p<0.05) was observed. Accordingly, up-regulation of miR-21 in the tumor is an important step in HCV-positive cirrhotic hepatocarcinogenesis and might result in concomitant down-regulation of PTEN and E-cadherin in favor of tumor promotion. Our data might be the first study that correlates miR-21, PTEN, and E-cadherin in different stages of HCV infection (from cirrhosis to HCC).
Keywords
miR-21; PTEN; E -cadherins; HCV; HCC
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