A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 147, Volume 89, Issue 1, October 2022, Page 5075-5082 PDF (848.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2022.261173 | ||||
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| Authors | ||||
Hager Ali Abu Elmagd   ; Eman Anwar Hassan; Moustafa Sarhan ; Nehal Abu-Elnag𝐚
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| Zoology department, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: In vivo, the zona pellucida (ZP) of the oocyte can bind to normally functional sperm. The ZP-sperm interaction is one of the final steps of natural selection during their journey in the female reproductive tract. Aim: In the current study, we evaluated the ability of the ZP of immature oocytes to harvest the fittest sperm. Method: We compared the embryological outcomes (i.e., rates of fertilization, cleavage, blastocyst formation, and high-quality blastocysts) of intracytoplasmic sperm injection (ICSI) using conventionally selected sperm (control group) and ZP-bound sperm (treated group). Results: Our results showed that there were no statistically significant differences for the ZP binding technique over the conventional sperm selection with respect to the rates of fertilization and cleavage. However; the rates of blastocyst formation and high-quality blastocysts were significantly higher in the treated group compared to the control group. Conclusion: These findings imply that the proposed technique (ZP-bound sperm) can serve as a cost-effective and natural sperm selection method that has the potential to improve the embryological and clinical outcomes of intracytoplasmic sperm injection (ICSI). | ||||
| Keywords | ||||
| Intracytoplasmic sperm injection (ICSI); zona pellucida (ZP); natural sperm selection; ZP-bound sperm; Acrosomal reaction (AR); Sperm-oocyte interaction | ||||
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