THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES
Hussien, H., Tag, H., Ahmed, E., Nabil, Z., El-Naggar, M. (2023). THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES. EKB Journal Management System, 79(79), 48-65. doi: 10.21608/ejz.2022.139640.1083
Hanan Tantawy Hussien; Hend M. Tag; Eman Ahmed; Zohour I. Nabil; Mohamed S. El-Naggar. "THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES". EKB Journal Management System, 79, 79, 2023, 48-65. doi: 10.21608/ejz.2022.139640.1083
Hussien, H., Tag, H., Ahmed, E., Nabil, Z., El-Naggar, M. (2023). 'THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES', EKB Journal Management System, 79(79), pp. 48-65. doi: 10.21608/ejz.2022.139640.1083
Hussien, H., Tag, H., Ahmed, E., Nabil, Z., El-Naggar, M. THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES. EKB Journal Management System, 2023; 79(79): 48-65. doi: 10.21608/ejz.2022.139640.1083

THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF TINOSPORA CORDIFOLIA LEAVES: IN VITRO AND IN VIVO STUDIES

Egyptian Journal of Zoology
Article 15, Volume 79, Issue 79, June 2023, Page 48-65  XML PDF (1.14 MB)
Document Type: Original Research Papers
DOI: 10.21608/ejz.2022.139640.1083
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Authors
Hanan Tantawy Hussien1; Hend M. Tag email orcid 1; Eman Ahmed2; Zohour I. Nabil1; Mohamed S. El-Naggarorcid 1
1Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt
2Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Abstract
Tinospora cordifolia has been used in Ayurvedic medicine to treat a number of diseases for a long time. The antioxidant and hepatoprotective activities of the ethanolic extract of T. cordifolia leaves (TC extract) were tested in the present study on the cultured rat hepatocytes and thioacetamide (TAA)-treated rats. For in vivo experiments, forty male Wistar rats were allotted randomly into eight groups (n=5): group “1” acted as the control group, group “2” represented the TAA-treated rats, groups “3-5” were treated with different doses of TC extract (50, 100, and 200 mg/kg body weight, respectively, orally/daily for 4 weeks); while groups “6-8” were TAA-treated rats that received 50, 100, and 200 mg, respectively, of TC extract/kg body weight (orally/daily for 4 weeks). The TC extract exhibited no marked cytotoxic activity against the cultured hepatocytes at IC50≤500 µg/mL (after 48 hours) in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The in vivo experiments revealed that the TC extract (at doses ≥100 mg/kg body weight) modulated significantly (P<0.05) the increase in the plasma aminotransferases and C-reactive protein levels, and lactate dehydrogenase activity, in the TAA-treated rats. In addition, the TC extract (at doses ≥50 mg/kg body weight) boosted significantly (P<0.05) the superoxide dismutase and catalase activities, and the glutathione content, in the hepatic tissues of the TAA-treated rats. Moreover, the histological results confirmed the hepatoprotective effects of the TC extract in the TAA-treated rats. In conclusion, the TC extract showed a hepatoprotective activity in the TAA-treated rats by its powerful antioxidant potential.
Keywords
Acute liver injury; Cultured hepatocytes; GC-MS analysis; MTT assay; Tinospora cordifolia
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