Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis
mosalem, M., fayed, S., ismail, Y., elfeky, H. (2022). Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis. EKB Journal Management System, 31(4), 45-52. doi: 10.21608/ejmm.2022.262685
manal mosalem; sahar mohamad fayed; yasser mohmoud ismail; hala mohammed elfeky. "Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis". EKB Journal Management System, 31, 4, 2022, 45-52. doi: 10.21608/ejmm.2022.262685
mosalem, M., fayed, S., ismail, Y., elfeky, H. (2022). 'Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis', EKB Journal Management System, 31(4), pp. 45-52. doi: 10.21608/ejmm.2022.262685
mosalem, M., fayed, S., ismail, Y., elfeky, H. Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis. EKB Journal Management System, 2022; 31(4): 45-52. doi: 10.21608/ejmm.2022.262685

Ascitic Fluid Micro RNA 155 as a Biomarker for Spontaneous Bacterial Peritonitis

Egyptian Journal of Medical Microbiology
Volume 31, Issue 4, October 2022, Page 45-52  XML PDF (447.66 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.262685
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Authors
manal mosalem email 1; sahar mohamad fayed2; yasser mohmoud ismail3; hala mohammed elfeky4
1clinical pathology, benha university of medicine, egypt
2clinical pathlogy department,faculty of medicine ,banha univerisity
3clinical pathology department, faculty of medicine, banha university
4hepatology, gastroenterology and infectious diseases department, faculty of medicine, banha university
Abstract
Background: Spontaneous bacterial peritonitis is a life-threatening complication in cirrhotic ascitic patients. Objectives: We aimed to evaluate ascitic fluid microRNA155 level as diagnostic markers of (SBP) in association with Leukocyte esterase test, MPV, PDW, ascitic fluid CRP and culure. Methodology: A cross sectional study included 64 patients with ascites admitted to the Hepatology, Gastroenterology and Infectious diseases Department, Benha University Hospital, Egypt, divided into two groups: SBP group (32 pts.) and non SBP group (32 pts.).50 ml. of ascitic fluid was obtained for assessment of CRP, Leukocyte esterase test, bacterial culture and miR-155 and 12 ml blood for the routine laboratory test. Results: The AUC of miRNA 155 level was acceptable for discrimination between both groups (AUC=0.959), with cut off values 1.14pg/ml. Sensitivity 93.8%, specificity 93,7%,  the cut-off value of ascitic fluid CRP was ≥12 ng/ml could predict SBP with sensitivity 81.3 % and specificity 81.2%  , the Leucocyte esterase test cut-off value  grade 1 and above could predict SBP with sensitivity 96.9 % , specificity of 84. %, accuracy of 90.6% ,with (AUC ) of  0.906,  MPV and PDW could  predict SBP with  cut of ≥10.5% and 14.3fl  , sensitivity of 84.4% and 59.4%% , and specificity of 84.4% and 62.5%% ,accuracy of 84.4 % and 60.9  %, the  combined usage of rapid bed side tests including leucocyte esterase strips, ascitic fluid CRP, MPV and PDW could predict SBP in almost all cases Conclusion: MiR-155 Leukocyte esterase test, MPV, ascitic fluid CRP and culure were good diagnostic markers of SBP.
Keywords
Spontaneous Bacterial Peritonitis; Micro RNA 155; Leukocyte esterase test; MPV; CRP and culture
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