Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients
raafat hamed, R., Bassyouni, R., fathi, H., badr, A., amer, M., dwedar, R. (2022). Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients. EKB Journal Management System, 31(4), 83-91. doi: 10.21608/ejmm.2022.262692
reham mohammad raafat hamed; Rasha Bassyouni; hanan fathi; amul m badr; marwa f amer; reham dwedar. "Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients". EKB Journal Management System, 31, 4, 2022, 83-91. doi: 10.21608/ejmm.2022.262692
raafat hamed, R., Bassyouni, R., fathi, H., badr, A., amer, M., dwedar, R. (2022). 'Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients', EKB Journal Management System, 31(4), pp. 83-91. doi: 10.21608/ejmm.2022.262692
raafat hamed, R., Bassyouni, R., fathi, H., badr, A., amer, M., dwedar, R. Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients. EKB Journal Management System, 2022; 31(4): 83-91. doi: 10.21608/ejmm.2022.262692

Cytomegalovirus and Epstein–Barr Virus Infection: Two Triggers flaring up Systemic Lupus Erythematosus Patients

Egyptian Journal of Medical Microbiology
Volume 31, Issue 4, October 2022, Page 83-91  XML PDF (355.12 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.262692
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Authors
reham mohammad raafat hamed email orcid 1; Rasha Bassyouniorcid 2; hanan fathiorcid 3; amul m badr4; marwa f amer4; reham dwedar1
1Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University, Egypt
2Fayoum Faculty of Medicine
3Rheumatology and Rehabilitation Department, Faculty of Medicine, Fayoum University, Egypt
4Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt
Abstract
Background: Systemic lupus erythematosus (SLE) is an autoimmune, multi-system, chronic inflammatory disease The effect of Cytomegalovirus(CMV) and Epstein-Barr virus(EBV) in triggering SLE has been investigated for many years. Objectives: To study the association of viral load of CMV-EBV in the serum of SLE patients’ with SLE disease parameters. Methodology: 48SLE patients and 40controls were enrolled. Disease activity was assessed using SLE disease activity index (SLEDAI). Quantitation of CMV and EBV-DNA in serum were detected by real-time polymerase chain reaction. Results: Patients were 91.8% females and 8.2%males. Mean age was 26.6±8.0 years, mean disease duration 4.5±3.1 years, and age at onset 22.1±7.8 years. 41.7% of SLE patients had CMV-DNA, 54.2% patients had EBV-DNA. Neither EBV-DNA nor CMV-DNA were found in the healthy controls. Copy numbers of CMV and EBV found in the serum of SLE patients were 26827±25879copies/μl, and 25309±22852copies/μl, respectively. Regarding SLEDAI; 83.3% showed high disease activity. Renal biopsy revealed that, 66.7% of the patients had lupus nephritis; 50% with CMV-DNA, and 56.25% with EBV-DNA. Regarding the association between CMV and EBV with different disease parameters in SLE patients; we found significant associations with: photosensitivity, Raynaud’s and thrombocytopenia (pvalue<0.05). EBV-DNA were significantly associated with: pyuria, oral ulcers, photosensitivity, vasculitis and involvement of nervous system (r=0.43, p=0.002), (r=0.36, p=0.01), (r=0.42, p=0.003), (r=-0.33, p=0.023), (r=0.32, p=0.029) respectively. Conclusions: A high incidence of CMV and EBV was detected in SLE patients with increased viral load. Disease activity of SLE patients is significantly higher in patients infected with CMV and EBV compared to non-infected.
Keywords
Systemic lupus erythematosus; Cytomegalovirus; Epstein-Barr virus SLEDA
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