Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt | ||||
| Microbial Biosystems | ||||
| Article 5, Volume 3, Issue 2, December 2018, Page 46-55 PDF (388.25 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mb.2018.26276 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| Endophytic fungi produce important bioactive primary and secondary metabolites. Researchers used L-Asparaginase for the treatment of Acute Lymphoblastic leukemia (ALL). Endophytic taxon, Lasiodiplodia theobromae (Pat.) Griff. and Maubl. has been isolated from Teucrium polium plant tissues collected from Saint Katherine Protectorate and was screened for its ability to produce L-Asparaginase qualitatively and quantitatively. The crude enzyme has been partially purified using gel-filtration Sephadex G-100 with an activity of 21.00 lMmL-1 min-1. The results revealed that L. theobromae (SCUF-TP2016) represents a rich source of bioactive metabolites that can be manipulated to produce L-Asparaginase which is a desirable novel analog for chemotherapy. | ||||
| Keywords | ||||
| Anti-leukemic; Enzymes; Saint Katherine Protectorate; gel filtration | ||||
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