Relationship of Sperm Characteristics, in Reference with DNA Fragmentation, with Fertility of Some Sheep Breeds in Egypt. | ||||
| Egyptian Journal of Sheep and Goats Sciences | ||||
| Article 5, Volume 12, Issue 1, April 2017, Page 1-10 PDF (558.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejsgs.2017.26325 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| SH. M. Shamiah; M. M. Al - Maghraby; A. M. Deghedy; A. A. El badawy | ||||
| Animal Production Research Institute, Agricultural Research Center, Egypt. | ||||
| Abstract | ||||
| ABSTRACT The objective of this study was to adopt an accurate in vitro test (sperm DNA damage) to predict fertility through ram spermatozoa. Semen was collected once a week for 8 collection weeks from six rams of Rahmani and Romanov breeds, 3 rams from each breed. Semen was evaluated for ejaculate volume, sperm concentration, progressive motility, livability and abnormality of spermatozoa. DNA damage test was applied with Halosperm kit. Results showed significant (P<0.05) individual differences in percentages of abnormality and DNA damage in both breeds as well as in percentages of progressive motility, livability and total sperm output in Romanove breed. Breed had a significant (P<0.05) effect only on ejaculate volume and total sperm output, being higher in Rahmani compared to Romanove breed. Sperm abnormality, sperm cell concentration and DNA damage showed an opposite trend with respect to breed effect. Rams showed the best semen quality in both breed had the highest conception rate (CR). Mean of CR was higher (P<0.05) in Rahmani than Romanov breed (88.38 vs. 78.79%, respectively). Percentages of progressive motility and livability of spermatozoa were negatively correlated (P<0.05) with DNA damage and positively with CR only in Rahmani breed. In both breeds, DNA damage had (P<0.01) a positive and strong correlation with percentage of abnormal sperm where it was r= 0.557 in Rahmani and r=0.840 in Romanov. In addition, CR had a positive correlation with sperm cell concentration in Rahmani and Romanove rams. The correlation between CR and sperm DNA damage was strong and negative (P<0.01) in both breeds (r=-0.699 for Rahmani and -0.795 for Romanov). In conclusion, the present study indicated the strong negative correlations between sperm DNA damage percentage of abnormal spermatozoa and conception rates especially for Romanov rams which showed lower CR. Thus, in vitro sperm DNA damage test can used for evaluating fertility, in accompany with standard semen analysis assays. | ||||
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