Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital
ALTabbakh, A., Saied, S., Shahat, A., ELMelouk, M. (2022). Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital. EKB Journal Management System, 31(4), 131-139. doi: 10.21608/ejmm.2022.263561
ALShaimaa Mahmoud ALTabbakh; Sara Ahmed Saied; Amany Shahat; Marwa seif ELMelouk. "Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital". EKB Journal Management System, 31, 4, 2022, 131-139. doi: 10.21608/ejmm.2022.263561
ALTabbakh, A., Saied, S., Shahat, A., ELMelouk, M. (2022). 'Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital', EKB Journal Management System, 31(4), pp. 131-139. doi: 10.21608/ejmm.2022.263561
ALTabbakh, A., Saied, S., Shahat, A., ELMelouk, M. Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital. EKB Journal Management System, 2022; 31(4): 131-139. doi: 10.21608/ejmm.2022.263561

Detection of Genes of Efflux Pumps (adeB, adeJ and adeG) in Tigecycline Resistant Acinetobacter baumannii Isolated from Benha University Hospital

Egyptian Journal of Medical Microbiology
Volume 31, Issue 4, October 2022, Page 131-139  XML PDF (588.48 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.263561
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Authors
ALShaimaa Mahmoud ALTabbakh email 1; Sara Ahmed Saied2; Amany Shahat3; Marwa seif ELMelouk4
1department of medical microbiology and immunology, faculty of medicine Benha University Egypt
2Clinical Pathology Department, National Liver Institute, Menoufia University, Egypt
3Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University
4Department of medical microbiology and immunology faculty of medicine Benha university, Egypt
Abstract
Background: Emerging of tigecycline resistant Acinetobacter baumannii (A. baumannii) is a critical global health problem as tigecycline is considered the last-line antibiotic for treatment of carbapenem resistant A. baumannii infections. Overexpression of efflux pumps is a leading mechanism of antibiotic resistance in A. baumannii. Objectives: Detection of the presence of three efflux pump genes; adeB, adeJ and adeG and determination of their expression level in Tigecycline Resistant A. baumannii collected from Benha University Hospital. Methodology: Thirty A. baumannii strains were collected and tested for antibiotic susceptibility. Presence of adeB, adeJ and adeG genes was detected by conventional PCR and their expression levels were assessed by RT-PCR. Results: Tigecycline susceptibility showed 60% (18/30) resistance and 40% (12/30) sensitivity. In tigecycline resistant strains, adeB gene was identified in 13/18 (72.2%) and adeJ gene in 12/18 (66.7%) with statistically significant association (P= 0.023). In tigecycline sensitive isolates adeB and adeJ genes were identified in 7/12 (58.3%) and 2/12 (16.7%) strains respectively. The adeG gene was not identified in any of the isolated strains. Combined adeB and adeJ genes were identified in 50% of tigecycline resistant isolates and only in 8.3% of (tigecycline sensitive) isolates with statistical significance difference (P= 0.018). Comparing adeB and adeJ gene expression revealed that transcription level was increased significantly and associated with tigecycline resistance. Conclusion: This study revealed wide spread of tigecycline resistant A. baumannii strains in Benha University Hospital and elucidated the significant role of (adeB and adeJ) genes in their emergence. 
Keywords
A. baumannii; Tigecycline resistance; RND efflux pump
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