COMPARATIVE STUDY BETWEEN POLYSACCHARIDES ISOLATED FROM OKRA AND PURSLANE | ||||
| Menoufia Journal of Agricultural Biotechnology | ||||
| Article 1, Volume 7, Issue 2, September 2022, Page 15-26 PDF (404.25 K) | ||||
| Document Type: original papers | ||||
| DOI: 10.21608/mjab.2022.263871 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| , Y.A.M. Ashoush1; , A.M.F Ali1; , M.M. Abozid1; , M.M.S. Asker2; , M.S.M. Salama3 | ||||
| 1Agriculture biochemistry department, Faculty of Agriculture, Menoufia University. | ||||
| 2Microbial Biotechnology Department. National Research Centre | ||||
| 3Mashtoul Elsouk Hospital | ||||
| Abstract | ||||
| Such investigation has as its goal to study polysaccharides from okra and purslane. The chemical composition of okra and Purslane is different. Okra contains 87.49% carbohydrates, 72.55% total soluble polysaccharides, 21% extraction percentage, against 79.51% carbohydrates, 63.0% total soluble polysaccharides, 17.5% extraction percentage for purslane. The infrared analysis reveald absorption in the region of 1725.18, 1732.38 and 1723.3 cm−1 for okra and purslane indicated the presence of CH2 group. The maximal absorption occurs at around 1626.54 cm−1 of Purslane reflects the polysaccharide property and relates to the stretching vibration of C=O of the N-acetyl group or protonated carboxylic acid. The peaks at 1598.44 and 1408.51 cm−1 of okra, 1588.32 and 1417.09 cm−1 of purslane were characteristic to the symmetric stretching of the COO- group, indicating that plants are acidic polysaccharides. The peak located at 1318.56 and 1244.31 cm−1 of okra, 1376.02 and 1322.88 cm−1 of purslane correlated with the ester sulphate groups' stretching vibration (S=O). The ingestion at 1144.54 and 1036.39 cm−1 of okra, 1024.08 cm−1of purslane in the range of 1200 – 1000 cm−1 which are anomeric regions, were attributed to the existence of C-O bonds in polysaccharides was shown by the compounds C-O-C and C-O-H that the monosaccharides in okra and purslane have α-pyranose ring. The presence of characteristic absorption at 893.03, 821.35 and 780.94 cm−1 of okra, 826.94, 778.58 and 719.28 cm−1 of purslane during anomeric area 950 – 700 cm−1 of plants were assigned to the coexistence of both α and β glycosidic connections. An estimated of the molecular weight of the polysaccharides of okra was 5.29 × 104 g/mol and purslane 1.25 × 104 g/mol. HPLC analysis of okra polysaccharides was indicated the monosaccharides of using a molar ratio manuronic acid, glucose, mannose, fucose, and arabinose at 0.52, 1.22, 0.96, 0.35, 1.0, respectively. Purslane polysaccharides contained manuronic acid, glucose, galactose and arabinose with molar ratio 0.36, 1.51, 1.0, 1.6, respectively. The 1H NMR spectra of okra and purslane polysaccharides clearly differs from each other in the pattern of resonances for their glycosyl anomeric and ring protons. The chromatogram of 1H NMR spectrum exhibited four peaks corresponding to the analysis of linkage and component reported in previous values. Among them, signals at 5.19–5.15 ppm were assigned to α-Arabinose residues, 4.87–4.91 ppm signals might represented the residues of α- Glucose pyranose. | ||||
| Keywords | ||||
| Polysaccharides; Okra; Purslane; Monosaccharides; Molecular weight; HPLC | ||||
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