The Effect of Zinc Oxide Nanoparticles and Their Withdrawal on the Pancreatic Acini of Adult Male Albino Rats: A Histological, Biochemical and Morphometric Study | ||||
| Egyptian Journal of Histology | ||||
| Article 32, Volume 46, Issue 4, December 2023, Page 1994-2016 PDF (21.37 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.161063.1765 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Reham Said Zaghloul1; Mostafa Mahmoud Abo El Ela2; Safaa Gomaa Takey El Din3; Silvia Kamil Sawires  4
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| 1histology and cell biology department, Alexandria Faculty of medicine | ||||
| 2histology and cell biology, Alexandria Faculty of medicine | ||||
| 3Histology and cell biology, Alexandria Faculty of Medicine | ||||
| 4Alexandria Faculty of medicine | ||||
| Abstract | ||||
| Introduction: Zinc oxide nanoparticles (ZnO-NPs) are one of metal nanoparticles that have broadly utilized in numerous fields. Nowadays, they are increasingly utilized in food as a preservative. Therefore, humans become more susceptible to their hazards via oral route. Objective: To assess the impact of different doses of ZnO-NPs on the pancreatic acini and the effect of their withdrawal. Materials and Methods: Thirty adult male albino rats were distributed into three equal groups at random. Group I acted as control. Group II (ZnO-NPs treated group): subdivided into two subgroups received ZnO-NPs in doses of 100 and 400 mg/kg body weight/day respectively for 28 days by oral gavage. Group III (withdrawal group): subdivided into two subgroups administered ZnO-NPs as in group II then left for 1 month without treatment. At the designated time, blood samples were collected for biochemical analysis. After scarification, pancreatic tissue was obtained and managed for light and electron microscopic studies. All the gained data underwent statistical analysis. Results: ZnO-NPs treated group showed dose-dependent pancreatic acinar cell damage. The cells exhibited vacuolations, rarefaction, dilated rough endoplasmic reticulum, nuclear condensation and mitochondrial degeneration which were more apparent in high doses. Moreover, biochemical studies showed a statistically significant rise in pancreatic and oxidant markers. In addition, morphometrical analysis revealed a statistically significant increase in area percentage of collagen deposition. However, ZnO-NPs withdrawal induced a great recovery concerning 100mg-treated rats but incomplete improvement was detected in 400mg-treated rats. Conclusion: ZnO-NPs induce structural and functional alterations on the pancreatic acini which are dose- dependent. When ZnO-NPs were withdrawn, these changes were reversible at low doses but partially improved with high doses. | ||||
| Keywords | ||||
| Microscopic examination; pancreatic acini; withdrawal; zinc oxide nanoparticles | ||||
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