Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections
Mostafa, N., Salah, M., Elashrey, A. (2022). Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections. EKB Journal Management System, 31(4), 141-149. doi: 10.21608/ejmm.2022.264208
Noha Mostafa; Mohammed Salah; Amira Elashrey. "Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections". EKB Journal Management System, 31, 4, 2022, 141-149. doi: 10.21608/ejmm.2022.264208
Mostafa, N., Salah, M., Elashrey, A. (2022). 'Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections', EKB Journal Management System, 31(4), pp. 141-149. doi: 10.21608/ejmm.2022.264208
Mostafa, N., Salah, M., Elashrey, A. Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections. EKB Journal Management System, 2022; 31(4): 141-149. doi: 10.21608/ejmm.2022.264208

Incomplete Hemolytic MRSA Strains Associated with Hemolysin and Panton-Valentine Leucocidin Virulence Genes as a Cause of Blood Stream Infections

Egyptian Journal of Medical Microbiology
Volume 31, Issue 4, October 2022, Page 141-149  XML PDF (449.96 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.264208
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Authors
Noha Mostafa email orcid 1; Mohammed Salah2; Amira Elashrey1
1Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
2Cardiovascular Medicine Department, Faculty of Medicine, Mansoura, Mansoura University, Egypt
Abstract
Background: Staphylococcus aureus (S. aureus) with the incomplete hemolytic phenotype (SIHP) is known for its dark hemolytic ring, which differs from the transparent S. aureus with complete hemolytic phenotype (SCHP). SIHP is recently linked to severe infections and antimicrobial resistance. Panton-Valentine leucocidin (PVL) and hemolysin are documented virulence factors for S. aureus infection. Objectives: We conducted this study to recognize methicillin-resistant S. aureus (MRSA) strains with SIHP phenotype and evaluate its association with the PVL and hemolysin in patients with bloodstream infections (BSIs). Methodology: Ninety-Three S. aureus isolates were recognized during the study period, and they were evaluated for resistance to thirteen antibiotics. Blood agar plates were used to culture these isolates, and they underwent ten passages for identification of their hemolytic phenotype. PVL and hemolysin genes were tested via polymerase chain reaction (PCR). Results: SIHP constituted 34.4% of the collected isolates. PVL positivity was significantly increased in SIHP (34.4% vs. 16.4% in SCHP). Also, SIHP was strongly associated with MRSA (62.5% vs. 39.3% of SCHP). In SIHP isolates, PVL positivity was associated with MRSA (55% vs. 0 % in MSSA) and increased resistance to Augmentin, cefoxitin, and gentamycin. Conclusion: The prevalence of SIHP is increasing among S. aureus. The prevalence of the PVL gene is higher in SIHP, and it is associated with the presence of MRSA.
Keywords
Staphylococcus aureus; Incomplete hemolytic phenotype; MRSA; Blood stream infections; pvl gene
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