Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM
Motawee, M., Hassan, F., Damanhory, A., Mohie, P., Khalifa, M., Elbastawisy, Y. (2022). Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM. EKB Journal Management System, 42(4), 329-343. doi: 10.21608/besps.2022.121674.1119
Mostafa E Motawee; Fatma Hassan; ahmed M Damanhory; Passant M Mohie; Mohamed Mansour Khalifa; Yasser M Elbastawisy. "Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM". EKB Journal Management System, 42, 4, 2022, 329-343. doi: 10.21608/besps.2022.121674.1119
Motawee, M., Hassan, F., Damanhory, A., Mohie, P., Khalifa, M., Elbastawisy, Y. (2022). 'Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM', EKB Journal Management System, 42(4), pp. 329-343. doi: 10.21608/besps.2022.121674.1119
Motawee, M., Hassan, F., Damanhory, A., Mohie, P., Khalifa, M., Elbastawisy, Y. Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM. EKB Journal Management System, 2022; 42(4): 329-343. doi: 10.21608/besps.2022.121674.1119

Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM

Bulletin of Egyptian Society for Physiological Sciences
Volume 42, Issue 4, October 2022, Page 329-343  XML PDF (948.31 K)
Document Type: Original Article
DOI: 10.21608/besps.2022.121674.1119
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Authors
Mostafa E Motawee1; Fatma Hassanorcid 2; ahmed M Damanhory3; Passant M Mohie4; Mohamed Mansour Khalifaorcid 2; Yasser M Elbastawisy email orcid 5
1Department of Histology and Cytology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
2Department of Medical Physiology, Faculty of Medicine, Cairo University, Egypt
3Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Egypt
4Department of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Egypt
5department of anatomy and embryology, faculty of medicine, mansoura university
Abstract
Background: Patients with diabetes mellitus are at significant risk for variable complications particularly, gastrointestinal tract disorders such as peptic ulcers. Omega-3 polyunsaturated fatty acids (PUFAs) and leptin can help tissue healing through their anti-inflammatory, antidiabetic, and antioxidant effects. This work aimed to compare the impact of ω-3 PUFAs and leptin on gastric ulcers as diabetic complications.
Methods: 36 rats were divided into negative control (n = 9) and Streptozotocin-induced type-II diabetes mellitus (n = 27) groups. The diabetic group was subdivided into three subgroups (n=9): diabetic control, diabetic with leptin, and diabetic with ω-3 PUFAs. A single oral indomethacin dose induced the ulcer by the end of the experiment. We assessed gastric mucosa gross appearance, histopathological parameters, and biochemical reactions.
Results: Chronic administration of leptin and ω-3 PUFAs remarkably improved gastric ulcer index, percentage ulcer protection, the expression of gastric GSH, CCK and e-NOS genes, and significantly decreased the expression of H+/K+-ATPase gene and COX-2 enzyme activity.
Conclusion: The current study found that leptin and ω-3 have antacid, anti-inflammatory, and antioxidant effects on indomethacin-induced gastric ulcers in diabetic rats, with a more potent effect of ω-3. We hypothesize that combined administration of both might have a synergetic anti-ulcer impact, supported by previously published studies.
Keywords
Indomethacin-induced gastric ulcer; omega 3; Leptin; DM and Glutathione
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