Possible Protective Effect of Each of Omega-3 PUFA and Leptin on Indomethacin-Induced Gastric Ulcer in Rats with type II DM | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Volume 42, Issue 4, October 2022, Pages 329-343 PDF (948.31 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2022.121674.1119 | ||
| Authors | ||
| Mostafa E Motawee1; Fatma Hassan2; ahmed M Damanhory3; Passant M Mohie4; Mohamed Mansour Khalifa2; Yasser M Elbastawisy* 5 | ||
| 1Department of Histology and Cytology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt | ||
| 2Department of Medical Physiology, Faculty of Medicine, Cairo University, Egypt | ||
| 3Department of Biochemistry, Faculty of Medicine, Al-Azhar University, Egypt | ||
| 4Department of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Egypt | ||
| 5department of anatomy and embryology, faculty of medicine, mansoura university | ||
| Abstract | ||
| Background: Patients with diabetes mellitus are at significant risk for variable complications particularly, gastrointestinal tract disorders such as peptic ulcers. Omega-3 polyunsaturated fatty acids (PUFAs) and leptin can help tissue healing through their anti-inflammatory, antidiabetic, and antioxidant effects. This work aimed to compare the impact of ω-3 PUFAs and leptin on gastric ulcers as diabetic complications. Methods: 36 rats were divided into negative control (n = 9) and Streptozotocin-induced type-II diabetes mellitus (n = 27) groups. The diabetic group was subdivided into three subgroups (n=9): diabetic control, diabetic with leptin, and diabetic with ω-3 PUFAs. A single oral indomethacin dose induced the ulcer by the end of the experiment. We assessed gastric mucosa gross appearance, histopathological parameters, and biochemical reactions. Results: Chronic administration of leptin and ω-3 PUFAs remarkably improved gastric ulcer index, percentage ulcer protection, the expression of gastric GSH, CCK and e-NOS genes, and significantly decreased the expression of H+/K+-ATPase gene and COX-2 enzyme activity. Conclusion: The current study found that leptin and ω-3 have antacid, anti-inflammatory, and antioxidant effects on indomethacin-induced gastric ulcers in diabetic rats, with a more potent effect of ω-3. We hypothesize that combined administration of both might have a synergetic anti-ulcer impact, supported by previously published studies. | ||
| Keywords | ||
| Indomethacin-induced gastric ulcer; omega 3; Leptin; DM and Glutathione | ||
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