Effects of Crystal Selenium Nanoparticles (Cys Se-NPS) on Carbofuran-induced biochemical, immunohistochemical and ultrastructure changes in the Thyroid Follicles of Adult Male Albino Rats | ||||
| Egyptian Journal of Histology | ||||
| Article 35, Volume 46, Issue 4, December 2023, Page 2052-2063 PDF (1.31 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.155372.1751 | ||||
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| Authors | ||||
Sahar Khalil   1; Omayma M Mahmoud2; Hala M.F. Mohammad3; Abeer A.K. Mohamed4
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| 1Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 2Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. | ||||
| 3Department of Clinical Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. Central Laboratory, Center of Excellence in Molecular and Cellular Medicine (CEMCM), Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 4Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| Introduction: Carbofuran, a broad-spectrum carbamate insecticide (endocrine disruptor) affects thyroid glands. Selenium-NPs is effective as selenite as a trace element for thyroid gland functioning while being less hazardous. Aim of wWork: The aim is to examine probable protecting effect of Cys Se-NPs on carbofuran induced rat thyroid glands damage. Materials and Methods: Forty adult male albino rats were divided randomly to four groups; control group, carbofuran group: received 2.4 mg/kg bw carbofuran, carbofuran & Cys Se-NPs group: received carbofuran as previous group in addition to 0.3 mg/kg bw Cys Se-NPs and finally Cys Se-NPs group: received Cys Se-NPs. Serum analysis of T3 and, T4 hormones and tissue GSH and MDA markers were evaluated. Thyroid sections of 5 μm thickness were prepared for H&E stains, Masson’s trichrome stains and detection of Ki67 antibodies immunohistochemical stain. Other thyroid specimens were processed for examination by transmission electron microscope. Results: Carbofuran group significantly revealed reduction in GSH and both T3, T4 and increase level of MDA. Thyroid follicles were highly disorganized and destructed, with follicular cells stratification and significant increase in epithelial thickness. Exfoliated cells were detected in the colloid with reduction in its volume. Carbofuran & Cys Se-NPs group revealed significant rise in GSH and T3, T4 and reduction in MDA level. Histological and ultrastructure abnormalities were improved significantly. Ki67 index was reduced by 3.69% compared to 62.4% in carbofuran group. Conclusions: Cys Se-NPs improved the changes induced by the toxic effects of carbofuran which can be a promising therapy against ROS produced by the carbofuran. | ||||
| Keywords | ||||
| Carbofuran; cys se-nps; ki67 index; MDA&GSH; rat | ||||
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