Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity
M. Hamed, M., S. Abdelftah, L. (2019). Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity. EKB Journal Management System, 23(1), 173-184. doi: 10.21608/ejabf.2019.26508
Moaz M. Hamed; Lamis S. Abdelftah. "Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity". EKB Journal Management System, 23, 1, 2019, 173-184. doi: 10.21608/ejabf.2019.26508
M. Hamed, M., S. Abdelftah, L. (2019). 'Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity', EKB Journal Management System, 23(1), pp. 173-184. doi: 10.21608/ejabf.2019.26508
M. Hamed, M., S. Abdelftah, L. Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity. EKB Journal Management System, 2019; 23(1): 173-184. doi: 10.21608/ejabf.2019.26508

Biosynthesis of gold nanoparticles using marine Streptomyces griseus isolate (M8) and evaluating its antimicrobial and anticancer activity

Egyptian Journal of Aquatic Biology and Fisheries
Article 13, Volume 23, Issue 1, January 2019, Page 173-184  XML PDF (502.64 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2019.26508
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Authors
Moaz M. Hamed; Lamis S. Abdelftah
Abstract
The implementation of actinomycetes for the biosynthesis of metal nanoparticles as a nature-friendly, safe and hopeful way is welcome due to its non-toxicity and naturalness. Out of nine actinomycetes isolates was isolated from sediment of the Suez Gulf, Egypt, only one isolate (M8) showed ability for biosynthesis of gold nanoparticles using extracellular supernatant. Isolate (M8) was selected and identified as Streptomyces griseus on the basis of cultural, morphological, and physiological properties, additionally 16S rRNA sequence. The gold nanoparticleswere confirmed using by Visible UV spectrophotometer, Fourier Transform Infrared (FTIR) Spectroscopy and Transmission Electron Microscopy (TEM). Au-Nps ranged from 19 to 28 nm in size and hexagonal in shape. Au-NPs synthesized by Streptomyces griseus isolate (M8) showed displayed a significant antimicrobial activity against gram positive bacteria Enterococcus faecalis 29212 (10 mm), gram negative bacteria Escherichia coli 19404, Pseudomonas aeruginosa 9027, Salmonella typhimurium 14028, Vibriofluvialis, and Vibrio damsel (10, 20, 28, 25 and 18 mm respectively), and yeast Candidaalbicans (18 mm) in well diffusion method. Moreover, gold nanoparticles exhibited a significant degree of anticancer activity against two different cancer cell lines Colon carcinoma cells (HCT-116) using 61.9 ug/well and breast carcinoma cells (MCF-7) using 46.6 ug/well.
Keywords
Gold nanoparticles; Streptomyces griseus; Actinomycetes; Antibacterial; Anticancer
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