Differential Gene Function and Physico-Chemical Properties of Sugar Beet Chromatin | ||||
| Alexandria Science Exchange Journal | ||||
| Article 6, Volume 32, OCTOBER- DECEMBER - Serial Number 4, December 2011, Page 422-428 PDF (643.87 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asejaiqjsae.2011.2652 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Seehy M. A1; Amira M. E. Khatab2 | ||||
| 1Dept. Genetics Fac. of Agric. Alexandria University. | ||||
| 2Dept. of Biology Fac. of Girls Education Univ. of Elmajmaah. Kingdom of Saudi Arabia. | ||||
| Abstract | ||||
| The present investigation aims at studying the possibility to use the physico-chemical properties of the purified chromatin as an indicator for transcriptional activity. In order to achieve such a purpose, five different breeding materials of sugar beet are selected and used. These materials are: 1, 2, 3, 4 and 5. Temperature of melting (Tm) estimated in 0C and hyperchromicity were estimated at 260 nm and 280 nm, as well as the chemical compositions of chromatin; DNA; Histones; non histones and total proteins. The chemical compositions, relative to DNA showed that different chemical compositions; temperature of melting; hyperchromicity, and repressed fraction of genome were obtained, giving a strong evidence that these molecular biomarkers might be used in breeding program of sugar beet. In conclusion the present investigation recommends the use of physico-chemical properties as a molecular biomarker to measure the transcriptional activity of plant materials. | ||||
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