Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions
Olatunde, O., Abidemi, O., Solomon, B., Bawa, J., Ibukun, O., Oluwatimilehin, I., Ayinke, A. (2022). Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions. EKB Journal Management System, 13(2), 113-120. doi: 10.21608/eajbsh.2022.265534
Omotosho Gbemiga Olatunde; Oladiti Rashidat Abidemi; Baruwa Abayomi Solomon; Joseph aphet Bawa; Oyelekan Esther Ibukun; Idowu Iyanuoluwa Oluwatimilehin; Akinpelu Bolajoko Ayinke. "Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions". EKB Journal Management System, 13, 2, 2022, 113-120. doi: 10.21608/eajbsh.2022.265534
Olatunde, O., Abidemi, O., Solomon, B., Bawa, J., Ibukun, O., Oluwatimilehin, I., Ayinke, A. (2022). 'Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions', EKB Journal Management System, 13(2), pp. 113-120. doi: 10.21608/eajbsh.2022.265534
Olatunde, O., Abidemi, O., Solomon, B., Bawa, J., Ibukun, O., Oluwatimilehin, I., Ayinke, A. Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions. EKB Journal Management System, 2022; 13(2): 113-120. doi: 10.21608/eajbsh.2022.265534

Assessment of Anti-inflammatory Activity of Prunus dulcis [Miller D.A. Webb (ALMOND)] SEED Aqueous Extract and Fractions

Egyptian Academic Journal of Biological Sciences, H. Botany
Article 10, Volume 13, Issue 2, December 2022, Page 113-120  XML PDF (632.82 K)
Document Type: Original Article
DOI: 10.21608/eajbsh.2022.265534
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Authors
Omotosho Gbemiga Olatunde1; Oladiti Rashidat Abidemi2; Baruwa Abayomi Solomon2; Joseph aphet Bawa2; Oyelekan Esther Ibukun2; Idowu Iyanuoluwa Oluwatimilehin2; Akinpelu Bolajoko Ayinke2
1Department of Medical Biochemistry, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria
2Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile ife, Osun State, Nigeria.
Abstract
Almonds (Prunus dulcis) seeds, from the Rosaceae family, have been reported to contain essential nutrients that reduced inflammation by lowering certain inflammatory markers. This study evaluated the anti-inflammatory activity of P.  dulcis (Almond seed) extract and fractions (n-hexane and aqueous). Dried almond seed (700 g) was pulverized and soaked in distilled water for 24 hrs. The aqueous extract was filtered and the filtrate was lyophilized to obtain the seed aqueous crude extract. The crude extract portion was dissolved in water, partitioned with n-hexane and separately concentrated to dryness to obtain the aqueous and n-hexane fractions. Anti-inflammatory activity of almond seed extract and fractions were carried out via erythrocyte membrane stabilization, antitryptic and protein denaturation inhibitory assays. Results obtained shows that n-hexane fraction of P. dulcis seed stabilized stressed erythrocyte maximally (18.70 ± 3.23%) at 2.00 mg/mL and aqueous fraction (16.72 ± 1.97%) at 0.5 mg/mL, while aqueous extract highest % stability was 17.73± 2.32% at 2.00 mg/mL. Also, n-hexane fraction inhibited trypsin better at higher concentrations while the aqueous fraction was at a lower concentration. However, both showed better anti-tryptic activity than aqueous crude extract.Moreover, maximum percentage protein inhibitory activities for diclofenac (800 µg/ml), aqueous fraction (800 µg/ml), n-hexane fraction (1600 µg/ml) and aqueous extract (1600 µg/ml) were 72, 51, 35 and 32 respectively.This study concludes that almond seed fractions showed better anti-inflammatory activity compared to their crude extract. Therefore, the isolation of active compounds from these fractions may provide a novel anti-inflammatory drug.
Keywords
Inflammation; Prunus dulcis; Membrane stabilization; Antitryptic; Protein denaturation
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