RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars
Abdein, M., Imrahim, A., Mohamed, S., Osman, S., Shamseldin, S., Maklad, M., Abdel-Salam, M., Qaoud, E. (2022). RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars. EKB Journal Management System, 49(2), 215-230. doi: 10.21608/ejoh.2022.155513.1206
Mohamed Abdein; Aly Mohamed Imrahim; Sayed Y. Mohamed; Samah O. Osman; Sahar A. M. Shamseldin; Mahmoud F. Maklad; Maha M. Abdel-Salam; EL-Sayed M. Qaoud. "RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars". EKB Journal Management System, 49, 2, 2022, 215-230. doi: 10.21608/ejoh.2022.155513.1206
Abdein, M., Imrahim, A., Mohamed, S., Osman, S., Shamseldin, S., Maklad, M., Abdel-Salam, M., Qaoud, E. (2022). 'RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars', EKB Journal Management System, 49(2), pp. 215-230. doi: 10.21608/ejoh.2022.155513.1206
Abdein, M., Imrahim, A., Mohamed, S., Osman, S., Shamseldin, S., Maklad, M., Abdel-Salam, M., Qaoud, E. RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars. EKB Journal Management System, 2022; 49(2): 215-230. doi: 10.21608/ejoh.2022.155513.1206

RAPD Markers are Associated with Self-incompatibility Characteristics as Related to the Number of Seeds per Fruit of Some Mandarin and Clementine Cultivars

Egyptian Journal of Horticulture
Volume 49, Issue 2, August 2022, Page 215-230  XML PDF (1.98 MB)
Document Type: Original Article
DOI: 10.21608/ejoh.2022.155513.1206
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Authors
Mohamed Abdein email orcid 1; Aly Mohamed Imrahim2; Sayed Y. Mohamed3; Samah O. Osman3; Sahar A. M. Shamseldin4; Mahmoud F. Makladorcid 5; Maha M. Abdel-Salamorcid 6; EL-Sayed M. Qaoud7
1Vegetable Breeding, Horticulture Inst., ARC, Giza, Egypt.
2Hort. Res. Inst., Agric. Res. Centre, Egypt
3Horticulture Research Institute, ARC, Giza 12619, Egypt
4Botany Department, Women’s College for Arts, Science and Education, Ain Shams University, Cairo 11566, Egypt
5Department of Horticulture, Faculty of Agriculture, Ain Shams University, Cairo 11241, Egypt
6Pomology Department, Faculty of Agriculture, Assiut University, Assiut, Egypt
7Horticultural Department, Faculty of Agriculture, Suez Canal University, 41522, Egypt
Abstract
The experiments were performed using (Nour, Fedela, Spinosa clementines, Kishu seedless, Balady, and Seedless mandarins) to determine the compatibility or incompatibility as related to the number of seeds per fruit and the genetic relation between them. For two consecutive seasons (2020 and 2021), the growth of the pollen tube in the styles following self-pollination has been analyzed using a fluorescence microscope to detect incompatibility properties. According to Nour and Fedela clementine cultivars are highly incompatible, while the other four cultivars are incompatible. After receiving pollen, Nour and Fedela clementines had very low pollen tubes that reached style. On the other hand, the highest rate was in the other four cultivars. The DNA of the six cultivars of mandarin and clementine investigated in this study was very similar. Balady mandarin and seedless mandarin appear to have very high similarities, while Fedela clementine and seedless mandarin appear to be very low. RAPD-PCR showed a specific molecule related to incompatibility such as (A-18) RAPD primer, at MW 361 bp. linked to the incompatibility of Nour and Fedela clementine c.vs.
Keywords
Self- incompatibility; Pollen tube growth; Fruit set; RAPD marker; Mandarins
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