Molecular and Phenotypic Analysis as A Diagnostic Tool of Candida Spp Infections Associated with COVD19 | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 11, Volume 14, Issue 2, December 2022, Page 135-142 PDF (418.99 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2022.265797 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nibras Salman Faraj1; Al-Dulaimi, Fahad K.Y.2; Rafea Zaidan Alsugmiany1 | ||||
| 1University of Tikrit-collage of science/department of biology. | ||||
| 2Northern Technical University/ Agricultural Technical College / Department of Animal Production Techniques. | ||||
| Abstract | ||||
| Several cases of oral candidiasis have recently been reported in patients with COVID-19, and this may lead to increased risks associated with morbidity and mortality. The current study aimed to evaluate the Molecular and phenotypic analysis as a diagnostic tool for Candida spp infections associated with COVD19. The study included 100 patients infected with the Coronavirus 19 who attended Al-Shirqat General Hospital, where yeasts were isolated from the oral cavity of those infected with the virus, by taking a swab and examining it in two ways, the KOH method and by culturing on a pre-prepared nutrient medium, the plates were incubated. The reactions of the RAPD (using 10 primers) technique were used for molecular diagnosis and analysis. The results of direct microscopic examination of a total of 100 samples showed that 35 samples were positive at a rate of 35%, while the number of negative samples was 65 samples at a rate of 65%. The results of the laboratory culture showed that there were 40 positive samples for growth, with a percentage of 40%, while the number of negative samples was 60 samples, and with a percentage of 60%, no growth.The results of RAPD-PCR indicators using 10 primers and the phase on 1% agarose gel showed 90 identification sites for the total primers, of which 89 were differentiated sites and one year site. Unique and absent, a total of 13 bundles for all prefixes, 11 unique and 2 absent bundles, and the total variance ratio was 99% for all prefixes The molecular sizes of the resulting bundles of the prefixes ranged between 100 -2000bp, where the highest number of packets was in primer P9, which gave 13 sites, while the lowest was in primer P10, which gave 3 sites, and the highest packets were in primer P5 with a total of 45 packets, and the lowest in primer P10 with a total of 12 packets, and also produced in primer P9 with 4 unique packets The lowest was in P1, P3, P5, P6, P8, with one beam for each primer, while the absent appeared only in P3, P2 primer s, respectively, with one beam for each primer. We can conclude the possibility of using the primers used in the technique of RAPD PCR as a diagnostic method for candidiasis in a more efficient and faster way than the endoscopic diagnosis in infections associated with Covid 19. | ||||
| Keywords | ||||
| RAPD-PCR; DNA; Candida spp; molecular; phenotyping | ||||
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