Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits
El-Aref, H., W., F., Nasr, M., Hemeida, A., Abouel-Khier, A. (2013). Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits. EKB Journal Management System, 44(3), 46-62. doi: 10.21608/ajas.2013.265999
Hamdy M. El-Aref; Fawaz, A. W.; M. I. Nasr; A. A. Hemeida; Alaa S. M. Abouel-Khier. "Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits". EKB Journal Management System, 44, 3, 2013, 46-62. doi: 10.21608/ajas.2013.265999
El-Aref, H., W., F., Nasr, M., Hemeida, A., Abouel-Khier, A. (2013). 'Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits', EKB Journal Management System, 44(3), pp. 46-62. doi: 10.21608/ajas.2013.265999
El-Aref, H., W., F., Nasr, M., Hemeida, A., Abouel-Khier, A. Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits. EKB Journal Management System, 2013; 44(3): 46-62. doi: 10.21608/ajas.2013.265999

Genetic Variability among Sugarcane Genotypes Based on TRAP Markers of Candidate Drought Genes and Some Agronomic Traits

Assiut Journal of Agricultural Sciences
Article 4, Volume 44, Issue 3, September 2013, Page 46-62  XML PDF (1 MB)
Document Type: Original Article
DOI: 10.21608/ajas.2013.265999
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Authors
Hamdy M. El-Aref1; Fawaz, A. W.2; M. I. Nasr3; A. A. Hemeida3; Alaa S. M. Abouel-Khier3
1Dept. of Genetics, Fac. of Agriculture, Assiut Univ., Assiut, Egypt
2Genetic Engineering Research Center
3Genetic Engineering and Biotechnology Res. Institute (G.E.B.R.I), Sadat City Univ., Egypt
Abstract
The study was focused on evaluation the performance of 12 sugarcane (Saccharum officinarum L.) genotypes under drought stress. In addition, the genetic variability among the tested genotypes were studied using Target Region Amplification Polymorphism (TRAP). markers based on polymorphism in Aquaporin (Aqua) and Dehydration Binding Factor (DBF) candidate drought genes.Stalk length, diameter, number, volume and Brix% were sharply affected by drought stress in comparison to the control treatment, however, highly significant differences between the tested genotypes were observed. Drought tolerance index (D.T.I) indicated that EI 8-129 followed by EI 24-3 were the most tolerant genotypes, while NCO310 and G2000 -79 were the most drought sensitive. Drought stress decreased chlorophyll contents in most genotypes except in EI 264-2, G84 – 47 and EI 266-2 the chlorophyll was increased.
Keywords
Sugarcane; Saccharum officinarum; genetic variability; TRAP markers; Aquaporin; Dehydration binding factor DBF; drought gene family; UPGMA cluster analysis
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