Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)
Ali, R., Kheir El Din, N., Abd-Rabou, A., Abu-seida, A., AMR, A. (2022). Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment). EKB Journal Management System, 68(4), 3229-3236. doi: 10.21608/edj.2022.137788.2104
Randa Ali; Nevine Kheir El Din; Ahmed Abd-Rabou; Ashraf Abu-seida; AHMED AMR. "Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)". EKB Journal Management System, 68, 4, 2022, 3229-3236. doi: 10.21608/edj.2022.137788.2104
Ali, R., Kheir El Din, N., Abd-Rabou, A., Abu-seida, A., AMR, A. (2022). 'Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)', EKB Journal Management System, 68(4), pp. 3229-3236. doi: 10.21608/edj.2022.137788.2104
Ali, R., Kheir El Din, N., Abd-Rabou, A., Abu-seida, A., AMR, A. Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment). EKB Journal Management System, 2022; 68(4): 3229-3236. doi: 10.21608/edj.2022.137788.2104

Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)

Egyptian Dental Journal
Article 3, Volume 68, Issue 4 - Serial Number 3, October 2022, Page 3229-3236  XML PDF (325.99 K)
Document Type: Original Article
DOI: 10.21608/edj.2022.137788.2104
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Authors
Randa Aliorcid 1; Nevine Kheir El Dinorcid 2; Ahmed Abd-Rabouorcid 3; Ashraf Abu-seidaorcid 4; AHMED AMR email orcid 2
1Faculty of Dentistry, University of Aden, Aden, Yemen
2Oral Medicine and Periodontology Department, Faculty of Dentistry, Ain-Shams University, Cairo, Egypt
3Hormones Department, Medicine and Clinical Studies Research Institute, National Research Center, Giza, Egypt
4Department of Surgery, Anesthesiology & Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Abstract
Background: The mode of harvesting autogenous bone grafts can affect the quality of harvested bone and viability. The present study was carried out to evaluate cellular viability, differentiation and proliferative power in autogenous bone graft harvested by auto chip maker versus milled bone block. Materials and Methods: 16 bone samples were harvested. In group I (n=8): auto chip maker (ACM) was used while in group II (n=8): bone blocks were harvested then milled. Flow cytometry, alkaline phosphatase activity, MTT assay, Alizarin Red Staining, and Real-Time PCR were performed Results: Group I had a significantly higher cell viability. MTT assay revealed a significant increase in cell proliferation in the ACM group. Both groups showed decrease in (RUNX2) gene expression over four days and increase in type 1 collagen alpha 1 and osteocalcin gene expression at 4th evaluation day with a statistical significant higher level of both markers in the ACM group. Conclusion: ACM group showed better osteoblasts viability, proliferation, differentiation, and calcified tissue formation.
Keywords
Auto chip Maker; Bone mill; Osteoblast viability; Osteocalcin
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