Algerian Capparis spinosa n-BuOH Extract Alleviates Diabetic Neuropathy Induced with Streptozotocin in Rats | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 65, Issue 132, December 2022, Page 519-538 PDF (1.47 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.156714.6790 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ibtissem Sakrani1; Souad Ameddah   2; Mouad Benrebaia3; Abdelahami Bouaroudge4; Ramazan Erenler5; Rachid Benkiniouar4; Ercan Cacan6; Ahmed menad 1
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| 1Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université des Frères Mentouri Constantine1, Algérie | ||||
| 2Laboratory of Biology and Environment, Faculty of Nature and Life Sciences,University Mentouri Brothers | ||||
| 3aLaboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université des Frères Mentouri Constantine1, Algérie | ||||
| 4Laboratoire des produits naturels d'origine végétale et de synthèse organique Université des Frères Mentouri Constantine, Algérie | ||||
| 5cDepartment of Chemistry, Faculty of Arts and Sciences, TokatGaziosmanpaşa University, 60240, Tokat, Turkey | ||||
| 6Department of Molecular Biology and Genetics, Gaziosmanpasa University, 60250 Tokat, Turkey | ||||
| Abstract | ||||
| Diabetic neuropathy (DN) is the most common diabetes complication. This study aimed to investigate the possible neuroprotective effects of the n-BuOH extract of Capparis spinosa (BECS) against DM-neuropathy induced with streptozotocin (STZ) and explore its phytoconstituents. From our results, BECS showed no toxicity against C6 brain cancer cell lines. The treatment of male Swiss albino rats with BECS (200 mg/kg) for a period of 28 days significantly attenuated the adverse effects of STZ-induced diabetes (60 mg/kg). A decrease in blood glucose was noted in the BECS-STZ group, associated with a significant (p<0.01) improvement in serum and brain insulin levels. The BECS- treated group showed a significant (p<0.01) improvement in serum C-peptide, serum AGE and serum CK-BB levels. BECS was also successful in restricting HbA1c in STZ-treated rats. Furthermore, a resurrection of neuronal membrane integrity was manifested in the BECS+STZ group, as evidenced by increased brain LDH activity and reduced brain MDA level. Treatment of STZ-treated rat with BECS was also able to significantly (p<0.01) regulate markers of brain dysfunction (AChE and BChE activities and glutamate ,dopamine levels). Additionally, treatment of rats with BECS efficacy restored the GSH level and GSH-system enzymes towards normal levels. Regarding the levels of (IL-6, TNF-α, and NO) produced by diabetogenic effects of STZ, the BECS successfully regulated these inflammatory mediators. From all these results, the neuroprotective effect of BECS could be due to its richness in polyphenols identified by LC-MS/MS (both positive and negative modes) analysis, mainly chlorogenic acid and rutin, as well as the synergistic interactions of all contents. | ||||
| Keywords | ||||
| Diabetic Neuropathy; Sreptozotocin; Capparis spinosa; LC-MS/MS analysis; Oxidative stress; Inflammation | ||||
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