Characteristics of Cucumber Mosaic Virus Isolates Infecting Cucurbits in Egypt | ||||
| Al-Azhar Journal of Agricultural Research | ||||
| Article 18, Volume 47, Issue 1, June 2022, Page 172-184 PDF (1.18 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajar.2022.266497 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Emad Sobhy Hamza  1; A. M. Al-Naggar1; H. M. El-Shabrawi2; I. H. Tolba1
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| 1Department of Agricultural Botany, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt | ||||
| 2Plant Biotechnology, Department Genetic Engineering and biotechnology, Division, National Research Center (NRC). | ||||
| Abstract | ||||
| Eight isolates of the Cucumber mosaic virus (CMV) were isolated from cucumber (Cucumis sativus) and zucchini (Cucurbita pepo) plants showing symptoms matched the CMV infection. The identity of these isolates was confirmed as CMV based on diagnostic hosts reaction, enzyme-linked immunosorbent assay (ELISA), and electron microscopy. The identification was confirmed at the molecular level. The obtained isolates were mechanically transmitted to diagnostic hosts which produced typical symptoms of CMV infection on each plant. Also, its easily transmitted by aphid (Aphis gossypii) in non-persistent manner. The virus could be transmitted through seeds of pumpkin, zucchini, and cucumber but not through seeds of melon. A variation in aggressiveness between obtained isolates were observed. Evaluating the disease reaction of some cultivated cucurbit types toward CMV artificially infection reveled that all the tested types were susceptible but differed in their susceptibility rate. | ||||
| Keywords | ||||
| Cucumber mosaic virus; ELISA; RT-PCR; seed transmission; reaction of cucurbits types | ||||
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