Pre-irradiation effects of ectoine on radiation-induced cardiotoxicity in female Swiss albino mice model | ||||
| Archives of Pharmaceutical Sciences Ain Shams University | ||||
| Article 1, Volume 6, Issue 2, December 2022, Page 169-180 PDF (592 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/aps.2022.148701.1094 | ||||
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| Authors | ||||
Sameh Nakhla   1; Basma Albehery2; Sana Shawky3; Salwa Lotfi4; Metwali Kotb5; Emad El-Bassiouni6; Eman El-Abd 1
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| 1Radiation sciences department, Alexandria University Medical Research Institute, Alexandria, Egypt | ||||
| 2Wadi El-Neel Hospital, Cairo, Egypt | ||||
| 3pathology Department, Alexandria University Medical Research Institute, Alexandria, Egypt | ||||
| 4Biological application Department, Nuclear Research Centre, Atomic Energy Authority, Cairo, Egypt | ||||
| 5Department of Medical Biophysics, Alexandria University Medical Research Institute, Alexandria, Egypt | ||||
| 6Pharmacology Department, Alexandria University Medical Research Institute, Alexandria, Egypt | ||||
| Abstract | ||||
| Background: Ectoine is a compatible solute that acts as a natural protectant. In mice model, a single post-irradiation ectoine dose showed protective effects by modulating both inflammatory and oxidative stress pathways. The current study aimed to explore the pre-irradiation effect(s) of ectoine on radiation-induced cardiotoxicity. Methods: Forty female Swiss albino mice (17.6-23.1 gm); controls (injected intraperitoneally for ten days with 0.2 ml saline), ectoine groups injected with 20 mg/kg of ectoine for ten days), irradiated groups (injected intraperitoneally for ten days with 0.2 ml saline then received six Gy whole body x-irradiation single dose), ectoine irradiated groups (injected with ectoine for ten days then irradiated). Animals were sacrificed on day seven, and 14 (five animals each). Hearts were examined for histological changes and immune-stained for Bax. Ectoine concentration in hearts was measured by HPLC. Serum cardiac troponin T, Total antioxidant capacity, and apoptosis-inducing factor were evaluated by mice ready to use ELISA kits. Results: Heart histological changes were documented in 40% of the 7- & 14-days post-irradiation. Ectoine concentrations (0.63×10^(-4) mg/mg of heart weight) were higher in ectoine groups than ectoine irradiated groups (0.011×10^(-4) mg/mg) 14-days post-treatment. Serum troponin T significantly differed between the 14 days groups (p = 0.032). Apoptosis inducible factor significantly increased in ectoine irradiated group (at 14 days) than those of control (p = 0.014), irradiated (p = 0.020), and ectoine (p = 0.033) groups. Bax showed strong to moderate immunostaining in ectoine and irradiated groups. Conclusions: Ectoine has pre-irradiation partial protective effects on heart cytotoxicity. | ||||
| Keywords | ||||
| Antioxidants; Apoptosis; Cardiotoxicity; Ectoine; Radiation; Troponin | ||||
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