Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients
Wafy, H., Abdelmalek, S., Mostafa, A. (2022). Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients. EKB Journal Management System, 4(2), 159-165. doi: 10.21608/jcvr.2022.267523
Hamed Wafy; Shymaa Abdelmalek; Alaa Eldin H. Mostafa. "Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients". EKB Journal Management System, 4, 2, 2022, 159-165. doi: 10.21608/jcvr.2022.267523
Wafy, H., Abdelmalek, S., Mostafa, A. (2022). 'Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients', EKB Journal Management System, 4(2), pp. 159-165. doi: 10.21608/jcvr.2022.267523
Wafy, H., Abdelmalek, S., Mostafa, A. Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients. EKB Journal Management System, 2022; 4(2): 159-165. doi: 10.21608/jcvr.2022.267523

Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients

Journal of Current Veterinary Research
Volume 4, Issue 2, October 2022, Page 159-165  XML PDF (799.88 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2022.267523
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Authors
Hamed Wafy* 1; Shymaa Abdelmalek2; Alaa Eldin H. Mostafa1
1Department of Microbiology, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
2Microbiology department, Faculty of Veterinary Medicine, Cairo University.
Abstract
Helicobacter infection is a major public health issue worldwide, about 4.4 billion individuals worldwide were confirmed to be infected. Helicobacter is a human pathogen that is transmitted from human to human and has a zoonotic relationship. Helicobacter gastritis, peptic ulcer, and perforation may lead to cancer. This study aims to try to isolate the Helicobacters from gastric biopsies and autopsies in human patients and animals (dogs and cats).
Methodology:105 gastric biopsies were collected from dyspeptic patients and 5 gastric autopsies were collected from dogs and cats. The samples were cultivated and characterized by microbiological and molecular tools (PCR).
Results: The results showed few harvested isolates (n=22) after characterization the confirmed Helicobacter isolates were 10 only from humans and animals.
Conclusion: The conclusion is the microbiological method is so costive, time-consuming, and less sensitive. The presence of accurate and rapid tests is so crucial for specific and sensitive detection of Helicobacters.
Keywords
Gastric biopsies; Helicobacter; Isolation; PCR
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