EFFECT OF SOYBEAN LECITHIN-BASED SEMEN EXTENDER ON FREEZABILITY AND FERTILITY OF RAHMANI RAM SPERMATOZOA | ||||
| Egyptian Journal of Sheep and Goats Sciences | ||||
| Article 6, Volume 9, Issue 1, April 2014, Page 1-8 PDF (192.8 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejsgs.2014.26759 | ||||
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| Authors | ||||
| Khalifa, E. I.; M. A. M. Abdel-Hafez | ||||
| Animal Production Research Institute, Sheep and Goat Research Department, Ministry of Agriculture, Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| ABSTRACT This experiment was designed to compare the protective action of soybean lecithin and egg yolk on cryopreservation and fertility of ram spermatozoa. The semen ejaculates were collected from three fertile rams, aged over 2.0 years and weighed round 65 kg, using artificial vagina. Two extenders were prepared, 1st served as the control and contain15% egg yolk with Tris-citric acid (TCEY).The second extender used 3.5 % soy lecithin to complete Tris-citric acid (TCSL). After primary evaluation of ejaculates, the semen samples were extended at the rate of 1 semen: 4 dilutes. The packed diluted semen was used to perform three experiments with TCEY and TCSL extenders. In the 1st experiment parameters such as progressive motility, viability and damaged acrosome were assessed after equilibration period at 5°C for 3 hours. Concerning the 2nd experiment, progressive motility, viability and abnormal acrosomes were evaluated after the freezing-thawing process. In the 3rd experiment a fertility test with post-thawing TCEY and TCSL extenders was performed. The results in the 1st experiment indicated that addition of 3.5% soylecithin increased (P>0.05) progressive motility, viability and reduced abnormal acrosomes of spermatozoa after equilibration period compared to 15% egg yolk extender. Although, TCEY and TCSL extenders in experiment 2nd showed non-significant differences in post-thawing sperm characteristics, the TCSL had improved post-thawing progressive motility, viability and rate of damaged acrosome of ram spermatozoa. In the 3rd experiment a better pregnancy rate (P<0.05) with TCSL (63.64 %) was observed compared to sperm preserved in TCEY (54.55 %). Based on these results, we conclude that use of a chemically defined, soy-based medium improves long-term motility and capacitation status of frozen–thawed ram' spermatozoa compared with cryopreservation in a traditional protection (egg yolk) extender. Furthermore, the study clarify that soybean lecithin could be able to increase proportions of viable frozen-thawed spermatozoa which reflected positively on fertility rates. | ||||
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