Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization | ||||
Mediterranean Aquaculture Journal | ||||
Article 3, Volume 4, Issue 1, January 2011, Page 12-17 PDF (1001.84 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/maj.2011.2676 | ||||
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Authors | ||||
Iman Abumourad1; Salah Meselhy2; Pin Nie3 | ||||
1Department of Hydrobiology, National Research Center | ||||
2Fish Health Department the WorldFish Center, Cairo, Egypt and | ||||
3State Key Laboratory of Freashwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province, China. | ||||
Abstract | ||||
The current study aimed to identify cytochrome c oxidase subunit 1 (ONCOX1) in Tilapia (Oreochromis niloticus) immunized by formalin-killed Flavobacterium columnarae. Suppressive subtractive hybridization (SSH) was utilized to construct a cDNA library and semi-quantitative RT-PCR analysis used to examine Oreochromis niloticus cytochrome c oxidase subunit 1 (COX1) gene expression. COX1 cDNA is composed of 1139 bps with a 1107 bps open reading frame, the predicted gene product is 369 amino acids with molecular weight of 40.16 kDa. The amino acid sequence revealed high identity with subunit 1 from Oreochromis mossambicus. Compared to ß-actin, the semi-quantitative RT-PCR revealed that ONCOX1 expressed in tissues of stimulated fish as up-regulated gene suggesting that this member of COX genes is probably involved in the general immune response against the pathogenic bacteria. | ||||
Keywords | ||||
tilapia; Cytochrome C oxidase; Protein prediction; Gene characterization; Tissue expression | ||||
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