Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic
A.Saeed, F., M.H.Abd-El-Moneem, K., S.Abd El – Magid, M., B.M. Fawaz, S. (2010). Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic. EKB Journal Management System, 41(2), 171-182. doi: 10.21608/ajas.2010.267891
Farag A.Saeed; Kenawy M.H.Abd-El-Moneem; Medhat S.Abd El – Magid; Said. B.M. Fawaz. "Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic". EKB Journal Management System, 41, 2, 2010, 171-182. doi: 10.21608/ajas.2010.267891
A.Saeed, F., M.H.Abd-El-Moneem, K., S.Abd El – Magid, M., B.M. Fawaz, S. (2010). 'Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic', EKB Journal Management System, 41(2), pp. 171-182. doi: 10.21608/ajas.2010.267891
A.Saeed, F., M.H.Abd-El-Moneem, K., S.Abd El – Magid, M., B.M. Fawaz, S. Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic. EKB Journal Management System, 2010; 41(2): 171-182. doi: 10.21608/ajas.2010.267891

Protein patterns in Relation to Virulence of Sclero-tium Cepivorum Berk. The Incitant of White Rot of Garlic

Assiut Journal of Agricultural Sciences
Article 10, Volume 41, Issue 2, May 2010, Page 171-182  XML PDF (569.99 K)
Document Type: Original Article
DOI: 10.21608/ajas.2010.267891
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Authors
Farag A.Saeed1; Kenawy M.H.Abd-El-Moneem2; Medhat S.Abd El – Magid3; Said. B.M. Fawaz4
1Plant Pathology Dept. Fac. Agric., Assiut Univ., Assiut, Egypt
2Plant Pathology Dept. Fac. Agric., Assiut Univ., Assiut, Egyp
3Plant Pathology Res. Institute, Agric., Research Center Giza Egypt
4Plant Pathology Res. Institute, Agric., Research Center Giza Egyp
Abstract
Six isolates of Sclerotium cepivorum Berk were isolated from naturally infected garlic plants collected from different localities of EL-Minya, Assiut and Sohag Governorates. Pathogenicty tests indicated that isolates No.2, 3 and 6 were highly pathogenic to garlic as compared with isolates No.1,4 and 5.
Protein of six isolates of S. cepivorum was compared by polyacrylamide gel electrophoresis (PAGE) and sodium dodecyel sulfate-polyacrylamide gel electophoresis (SDS-PAGE). Protein profiles separated by PAGE, isolate No. 1 showed the highest number of bands (20 bands), while isolate No. 4 showed the lowest number (15 bands). The number of bands of other isolates was 16 or 17 bands. Protein profiles separated by SDS-PAGE, isolate No. 5 showed the highest number of bands (19 bands) while isolate No. 3 showed the lowest number of bands (6 bands). The other isolates showed a number of bands ranged from 13 to 17 bands. On the basis of electrophoretic dissimilarities among protein banding paterns, isolates were grouped by claster analysis and the results were experessed as phenograms. Grouping the isolates based on PAGE analysis was associated with geographic of isolates,however, grouping the isolates based on SDS-PAGE was associated with virulence of isolates
Keywords
Scleratium cepirum; electrophoresis; protein patterns
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