Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution
Abdelfattah, P., Mostafa, M., Mohamed, E. (2022). Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution. EKB Journal Management System, 9(4), 699-705. doi: 10.21608/adjg.2022.45819.1303
Panseh W. Abdelfattah; Mohamed H. Mostafa; Eman AR. Mohamed. "Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution". EKB Journal Management System, 9, 4, 2022, 699-705. doi: 10.21608/adjg.2022.45819.1303
Abdelfattah, P., Mostafa, M., Mohamed, E. (2022). 'Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution', EKB Journal Management System, 9(4), pp. 699-705. doi: 10.21608/adjg.2022.45819.1303
Abdelfattah, P., Mostafa, M., Mohamed, E. Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution. EKB Journal Management System, 2022; 9(4): 699-705. doi: 10.21608/adjg.2022.45819.1303

Evaluation of the Effect of Green Tea Extracts on Periodontal Ligament Fibroblasts Viability versus Hank's Balanced Salt Solution

Al-Azhar Dental Journal for Girls
Article 17, Volume 9, Issue 4, October 2022, Page 699-705  XML PDF (722.97 K)
Document Type: Original Article
DOI: 10.21608/adjg.2022.45819.1303
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Authors
Panseh W. Abdelfattah email 1; Mohamed H. Mostafa2; Eman AR. Mohamed3
1Dentist at Alzahraa University Hospital
2Assistant Professor, Head of Pedodontics and oral Health Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt
3Lecturer of Pedodontics and Oral Health Faculty of Dental Medicine for girls Al-Azhar University, Cairo, Egypt.
Abstract
Purpose: To assess the effect of Green Tea Extract (GTE) on the periodontal ligament fibroblasts viability comparing to Hank’s Balanced Salt Solution (HBSS). Materials and methods: Periodontal ligament fibroblasts were obtained from 30 freshly extracted sound premolars and cultured in Dulbecco’s Modified Eagle Medium (DMEM). Cell viability was determined by storing the cells in Hank’s balanced salt solution (Group I), Green tea extract (Group II) and fresh Dulbecco’s Modified Eagle Medium (DMEM) (Group III) as positive control, for 1,3,6,12,24 and 48h at 37oC using MTT assay (3[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide). Results: Statistical analysis was performed by one-way analysis of variance (ANOVA) and post hoc tests.The findings of this study revealed that there was no significant difference between groups at 1h and 3h time intervals, while GTE showed considerably better protective effect than HBSS at 6 hrs. (p < /em> = 0.002), at 12 hrs. (p < /em> = 0.001), 24 hrs. (p = 3.38E-03) and 48 hrs. (p < /em> = 1.65E-03). Conclusion: Within the boundaries of the present study, it showed that GTE had higher protective effect on periodontal ligament fibroblasts than HBSS and hence can be used as alternative medium to HBSS.
Keywords
Avulsion; Green Tea Extract; cell viability; Hank’s Balanced Salt Solution
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