Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin
Hassan, T., Maher, M., El Karmoty, A., Ahmed, Z., Ibrahim, M., Rizk, H., Tolba, A. (2022). Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin. EKB Journal Management System, 68(1), 64-79. doi: 10.21608/vmjg.2022.268164
Taghreed Hassan; Mohamed Maher; Amr F. El Karmoty; Zainab S. O. Ahmed; Marwa A. Ibrahim; Hamdy Rizk; Ayman Tolba. "Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin". EKB Journal Management System, 68, 1, 2022, 64-79. doi: 10.21608/vmjg.2022.268164
Hassan, T., Maher, M., El Karmoty, A., Ahmed, Z., Ibrahim, M., Rizk, H., Tolba, A. (2022). 'Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin', EKB Journal Management System, 68(1), pp. 64-79. doi: 10.21608/vmjg.2022.268164
Hassan, T., Maher, M., El Karmoty, A., Ahmed, Z., Ibrahim, M., Rizk, H., Tolba, A. Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin. EKB Journal Management System, 2022; 68(1): 64-79. doi: 10.21608/vmjg.2022.268164

Regenerative therapy of rabbit ear cartilage using adipose derived stem cells and platelet rich fibrin

Veterinary Medical Journal (Giza)
Article 5, Volume 68, Issue 1, 2022, Page 64-79  XML PDF (1.08 MB)
Document Type: Original Article
DOI: 10.21608/vmjg.2022.268164
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Authors
Taghreed Hassan* 1; Mohamed Maher1; Amr F. El Karmoty1; Zainab S. O. Ahmed2; Marwa A. Ibrahim3; Hamdy Rizk1; Ayman Tolba1
1Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
2Cytology and Histology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
3Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Abstract
The objective of the current study is to evaluate using of platelet-rich fibrin (PRF) or adipose-derived stem cells (ADSCs) for the therapy of rabbit ear cartilage defects. Nine adult New Zealand white rabbits were divided into three groups. The rabbit ears developed two defects in the cartilage. Phosphate-buffered saline (PBS) was injected sub- perichondrially into Group I, adipose-derived MSCs (ADMSCs) were injected into Group II, and platelet-rich fibrin (PRF) was injected into Group III in the defective ear. After 4 weeks, the auricular defect was examined morphologically, histologically, and immunohistochemically. In addition, the gene expression of the collagen type II (Col II) and aggrecan. Anatomically, the auricles of all treatments seemed to be fully healed with smooth surfaces. Histopathologically, a small region of immature cartilage was seen in the control positive group's defects. While the group treated with PRF and ADSCs demonstrated the normal characteristics of new cartilage development, including mature chondrocytes within their lacunae and rich extracellular matrix (ECM). The PRF-treated group responded to Masson's trichrome and orcein stains, while produced a faint staining in the case of the control positive and ADMSC groups. Immunohistochemically, there was a strong positive S100 expression in ADSCs and PRF but mild reaction in the control group. The expression of relative col II and aggrecan was substantially highest in PRF (± 0.72 and ± 0.57, respectively). While, Control positive and ADMSC groups recorded (± 0.41 : ± 0.21, ± 0.6 : ± 0.44) respectively. The PRF had the highest rate of proliferation and chondrogenic potential when compared to the ADMSCs and control groups.
Keywords
Auricular cartilage defects; Adipose stem cells; Platelet rich fibrin; Rabbit
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