Radiation-induced effects on genes regulating DNA Repair and Cell Cycle in breast cancer patients.

Nakhla, Sameh Fawzy; Khafaga, Rana; Gaber, Marwa; Aref, Tarek; Anwar, Medhat; Saadi, Shaza; Mohamed, Esraa; Gad, Esraa; Hussien, Amr; Ismail, Abdelsalam; Moussa, Sawsan

doi:10.21608/zumj.2022.165257.2649

	Radiation-induced effects on genes regulating DNA Repair and Cell Cycle in breast cancer patients.
Zagazig University Medical Journal
Articles in Press, Accepted Manuscript, Available Online from 03 November 2022
Document Type: Original Article
DOI: 10.21608/zumj.2022.165257.2649
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Authors
Sameh Fawzy Nakhla ¹; Rana Khafaga²; Marwa Gaber³; Tarek Aref⁴; Medhat Anwar⁵; Shaza Saadi⁶; Esraa Mohamed⁷; Esraa Gad⁷; Amr Hussien⁸; Abdelsalam Ismail⁹; Sawsan Moussa¹⁰
¹Radiation Sciences department, Medical Research Institute (MRI), Alexandria University, Alexandria, Egypt.
²Department of Biochemistry, Medical Research Institute, Alexandria University.
³Department of Eperimental and Clinical internal Medicine, Medical Research Institute, Alexandria University.
⁴Department of Radiology, Medical Research Institute, Alexandria University
⁵Department of Experimental and Clinical Surgery, Medical Research Institute, Alexandria University
⁶Department of Radiology and Medical Imaging, Faculty of Medical Applied Sciences, Al-Azhar University- Palestine
⁷Department of Radiology, faculty of Applied Medical Science, October 6 University.
⁸Department of Cancer management and research, Medical Research Institute, Alexandria University
⁹Department of clinical oncology, Faculty of Medicine, Alexandria University
¹⁰Department of Radiation Science, Medical Research Institute, Alexandria University
Abstract
Background: Ionizing radiation is a major DNA damaging agent. One of the most common sources of exposure is through medical diagnostics or treatments such as cancer radiotherapy. Aim: This study aims to assess the radiation-induced changes in the expression of DNA repair and cell cycle regulation genes (POLH, PCNA, DDB2, and XPC) in the blood of breast cancer patients and to evaluate their potential as predictive biomarkers for treatment responses. Methods: In this study, the levels of circulating PCNA, POLH, XPC, and DDB2 were evaluated in 51 females: 31 cancer patients and 20 healthy volunteers as a control group. The genes were extracted from whole blood samples and cDNA was synthesized; RT-qPCR was used to assay the expression pattern of these genes. Results: Circulating DDB2 showed a significant difference in the relative expression among the three studied groups. Also, there was a significant difference in the relative expression of circulating DDB2 in the pre-radiotherapy and post-radiotherapy groups relative to the control group. Also, relative to the control group, the circulating levels of the four genes were higher in the pre-radiotherapy group to about double that in the post-radiotherapy group. The four genes combination was capable to discriminate the pre-radiotherapy group from the post-radiotherapy group significantly. Conclusion: The relative expression of these four genes as radiation-responsive genes that are involved in cell cycle regulation and DNA repair could be changed due to radiotherapy. Also, their circulating levels in breast cancer patients could be promising predictive biomarkers for radiotherapy responses.
Keywords
Breast Cancer; radiation-responsive genes; radiotherapy


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