Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.
Elbagory, G., Mahmoud, A., ELhabashy, R. (2022). Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.. EKB Journal Management System, 43(1), 60-64. doi: 10.21608/bvmj.2022.160161.1585
Gabr Fikry Elbagory; Ayman Hamed Mahmoud; Rawan ELhabashy. "Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.". EKB Journal Management System, 43, 1, 2022, 60-64. doi: 10.21608/bvmj.2022.160161.1585
Elbagory, G., Mahmoud, A., ELhabashy, R. (2022). 'Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.', EKB Journal Management System, 43(1), pp. 60-64. doi: 10.21608/bvmj.2022.160161.1585
Elbagory, G., Mahmoud, A., ELhabashy, R. Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.. EKB Journal Management System, 2022; 43(1): 60-64. doi: 10.21608/bvmj.2022.160161.1585

Isolation and identification of Foot and Mouth Disease Virus strains circulate in Egypt during 2021-2022 outbreaks.

Benha Veterinary Medical Journal
Volume 43, Issue 1, October 2022, Page 60-64  XML PDF (999.55 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2022.160161.1585
View on SCiNiTO View on SCiNiTO
Authors
Gabr Fikry Elbagory1; Ayman Hamed Mahmoud2; Rawan ELhabashy email 2
1faculty of veterinary medicine mostohor
2Biotechnology Research unit, Animal Health Research Institute, Dokki 12618, Agricultural Research Center, Giza 12619, Egypt.
Abstract
Foot-and-mouth disease (FMD) is an acute viral animal disease; that causes huge economic consequences to the livestock sector. Totally, 118 samples (Epithelial Tissue (n = 38), Vesicles (n = 30), Heart (n = 31), Tongue (n = 9), Oral swabs (n = 10), and 232 samples (Epithelial Tissue (n = 108), Vesicular fluids (n = 36), Heart (n = 28), Tongue (n = 24), Oral swabs (n = 36) collected from suspected animals during FMD outbreak investigations in 2021, and 2022, respectively, constituted the material of the study. This study aimed to detect the Foot-and-mouth disease viruses (FMDVs) and their serotype using qRT-PCR, and antigen detection using ELISA, followed by virus isolation on BHK-21 cells then identification. The field samples were analyzed by FMDV screening primers/probes then the virus serotype-specific primers/probes revealing 57/118 (48.30%) during 2021, and 158/232 (68.10%) during 2022 positive samples with qRT-PCR and categorized into serotype O 46/57(80.70%), 90/158(56.96%) with the highest prevalence, followed by serotype A 7/57(12.28%), 58/158(36.70%), serotype SAT-2 4/57(7%), 10/158(6.32%) during 2021, and 2022, respectively. FMD antigen detection ELISA was performed giving results with 45/118(38.14%) in 2021, and 120/232(51.72%) in 2022. The virus is propagated successfully in 4/10 (40%) in 2021, and 6/17(35.29%) in 2022 samples. Results illustrated in the present study help in general detection of FMDV serotypes currently circulating in Egypt, and aid in understanding of current status of epidemiological aspect of the disease to construct efficient control plan.
Keywords
FMDV serotypes; Real-time RT-PCR; Diagnosis; FMD status
Statistics
Article View: 337
PDF Download: 203