In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent
Ali, D., Abdel-Rahman, T., Abo-hagger, A., Ahmed, M. (2019). In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent. EKB Journal Management System, 59(2), 399-411. doi: 10.21608/ejbo.2019.5923.1247
Dalia M.I. Ali; Tahany M. Abdel-Rahman; Amel A. Abo-hagger; Mona S. Ahmed. "In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent". EKB Journal Management System, 59, 2, 2019, 399-411. doi: 10.21608/ejbo.2019.5923.1247
Ali, D., Abdel-Rahman, T., Abo-hagger, A., Ahmed, M. (2019). 'In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent', EKB Journal Management System, 59(2), pp. 399-411. doi: 10.21608/ejbo.2019.5923.1247
Ali, D., Abdel-Rahman, T., Abo-hagger, A., Ahmed, M. In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent. EKB Journal Management System, 2019; 59(2): 399-411. doi: 10.21608/ejbo.2019.5923.1247

In vitro and In vivo Assessment of Banana Peel Powder as an Aflatoxins Biosorbent

Egyptian Journal of Botany
Article 9, Volume 59, Issue 2, August 2019, Page 399-411  XML PDF (1.79 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2019.5923.1247
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Authors
Dalia M.I. Ali email 1; Tahany M. Abdel-Rahman1; Amel A. Abo-hagger2; Mona S. Ahmed2
1Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt
2Regional Center for Food and Feed, Agriculture Research Center, Giza, Egypt
Abstract
SURVEY of fungi in raw peanut seeds by washing technique method resulted in isolation of nine fungal species. The aflatoxigenic Aspergillus flavus was the most frequently isolated species in the isolation plates. Molecular identification confirms that it is identical to A. flavus available in database in NCBI GenBank with similarity of 100%. The aflatoxins (AFs) produced in the fermentation medium of A. flavus were extracted and estimated by HPLC to be contain B1, B2, G1, G2 AFs. Banana peel powder, prepared from samples collected from juice markets, proved to be of high adsorption capacity to aflatoxins in concentration depending manner of either peel or aflatoxins.
In vivo histopathological studies using 6 albino rats groups, an alteration in rats treated with single aflatoxin concentrations (G2 or G3) such as inflammation, fibrosis, expanded portal tract and degeneration of hepatocytes in liver and expanded glomeruli, vacuolation & fatty degeneration in renal tubules were detected in kidney. However, banana peel treated singly showing normal hepatocytes and central vein in liver as well as normal tubules and glomeruli in kidney. Co-addition of AFs with banana peel powder to rat diet showing significant reduction in the harmful effect of AFs added singly in liver and kidney tissues. This study indicated also that the liver was highly affected by AFs than kidney.


Keywords
Aflatoxins; banana peel; albino rats; Adsorption; histopathology; agricultural wastes
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