Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies
Fakher El Deen, R., El-Naggar, S., El-Nahass, E., El-Said, K. (2022). Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies. EKB Journal Management System, 8(4), 264-274. doi: 10.21608/jbaar.2022.268775
Rania Shams El Deen Fakher El Deen; Sabry Ali El-Naggar; Eman El-Nahass; Karim El-Said. "Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies". EKB Journal Management System, 8, 4, 2022, 264-274. doi: 10.21608/jbaar.2022.268775
Fakher El Deen, R., El-Naggar, S., El-Nahass, E., El-Said, K. (2022). 'Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies', EKB Journal Management System, 8(4), pp. 264-274. doi: 10.21608/jbaar.2022.268775
Fakher El Deen, R., El-Naggar, S., El-Nahass, E., El-Said, K. Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies. EKB Journal Management System, 2022; 8(4): 264-274. doi: 10.21608/jbaar.2022.268775

Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies

Journal of Bioscience and Applied Research
Volume 8, Issue 4, December 2022, Page 264-274  XML PDF (1.23 MB)
Document Type: Original Article
DOI: 10.21608/jbaar.2022.268775
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Authors
Rania Shams El Deen Fakher El Deen1; Sabry Ali El-Naggar2; Eman El-Nahass2; Karim El-Said email 3
1Nutrition and Food Science, Faculty of Specific Education, Tanta University, Egypt
2Zoology Department, Faculty of Science, Tanta University, Egypt
3Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Egypt
Abstract
Background: Natural products demonstrated potential for use in medicine and pharmaceuticals. Objective: This study evaluated the phytochemical composition of Urtica sp. leaves extract (USLE), and its in vitro and in vivo antitumor effects. Methods: HepG-2 and MCF-7 cell lines were used to evaluate the IC50, apoptosis, and cell cycle analysis of USLE. Forty female CD-1 mice were equally divided as follows; Gp1 was control; Gp2, Gp3, and Gp4 were inoculated with 1 × 106 of Ehrlich ascitic carcinoma (EAC)-cells/mouse. Then, Gp3 had injected with Cisplatin (2 mg/kg) intraperitoneally (i.p). Gp4 had injected with USLE (100 mg/kg) i.p. as in Gp3. Total tumor volume, total tumor cell count, and live and dead EAC-cells were determined, also biochemical and histopathological investigations were evaluated. Results: Data showed that the USLE had cytotoxic and antitumor effects against the HepG-2, MCF-7, and EAC-bearing mice. Biochemical and histopathological investigations showed an improvement in the liver and kidney tissues upon treatment of EAC-bearing mice with USLE. Conclusion: The results showed that the USLE can stop tumor growth and cause tumor cells to die.
Keywords
Urtica sp; GC-MS; Antioxidants; Antitumor; EAC-bearing mice
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