Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere
Batroukha, Y., Nour El Dein, M., Abou-Dobara, M., El-Sayed, A. (2022). Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere. EKB Journal Management System, 12(1), 124-131. doi: 10.21608/sjdfs.2022.269835
Yara Ahmed Fouad Batroukha; Mahmoud Metwally Nour El Dein; Mohamed I. Abou-Dobara; Ahmed K.A. El-Sayed. "Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere". EKB Journal Management System, 12, 1, 2022, 124-131. doi: 10.21608/sjdfs.2022.269835
Batroukha, Y., Nour El Dein, M., Abou-Dobara, M., El-Sayed, A. (2022). 'Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere', EKB Journal Management System, 12(1), pp. 124-131. doi: 10.21608/sjdfs.2022.269835
Batroukha, Y., Nour El Dein, M., Abou-Dobara, M., El-Sayed, A. Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere. EKB Journal Management System, 2022; 12(1): 124-131. doi: 10.21608/sjdfs.2022.269835

Production and Optimization of Gelatinase Producing Lentzea sp. Strain Isolated from the Soil Rhizosphere

Scientific Journal for Damietta Faculty of Science
Volume 12, Issue 1, 2022, Page 124-131  XML PDF (342.26 K)
Document Type: Short communications
DOI: 10.21608/sjdfs.2022.269835
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Authors
Yara Ahmed Fouad Batroukha email 1; Mahmoud Metwally Nour El Dein2; Mohamed I. Abou-Dobara3; Ahmed K.A. El-Sayed3
1Microbiology, Science, Damietta, New Damietta, Egypt
2Botany Dept., Faculty of Sci., Damietta Univ., New Damietta., P.O. 34517, Egypt
3Botany Dept., Faculty of Sci., Damietta Univ., New Damietta, P.O. 34517, Egypt
Abstract
Proteases are the major enzymes that have been used widely in health, food and nutritional supplement fields. Rare actinobacteria are considered a new resource for proteolytic enzyme activity. Rare actinobacteria are used to be isolated from marine and have been studied on restricted limit. The strain used in this study was identified as Lentzea sp. which successfully isolated from the soil at Faculty of Science, Damietta, Egypt. It was subjected to gelatin liquefaction as primary screening for gelatinolytic activity. Based on this study, the best gelatinolytic (gelatinase) activity was noticed within 8 days at 37oC (17.59 U/ml ±0.06). The maximum activity was obtained at pH 9 (28.33 U/ml ±0.04) and at agitation speed 150 rpm (29.10 U/ml ±0.04). Upon carbon and nitrogen source optimization, the basal medium of combination between gelatin and peptone produced maximal gelatinase activity (29.12 U/ml ±0.06) and it was found that 10% sucrose was the best carbon source that produced maximum gelatinase activity at (30.17 U/ml ±0.05).
Keywords
Lentzea sp; actinomycetes; gelatinase activity; optimization
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