The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics
Zayed, M., Abd El-Fattah, D., Abbas, R., El-Bordeny, N., Edris, S. (2023). The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics. EKB Journal Management System, 31(1), 29-40. doi: 10.21608/ajs.2022.155161.1489
Mona Zayed; Dalia Abd El-Fattah; Rateb Abbas; N. El-Bordeny; Sherif Edris. "The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics". EKB Journal Management System, 31, 1, 2023, 29-40. doi: 10.21608/ajs.2022.155161.1489
Zayed, M., Abd El-Fattah, D., Abbas, R., El-Bordeny, N., Edris, S. (2023). 'The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics', EKB Journal Management System, 31(1), pp. 29-40. doi: 10.21608/ajs.2022.155161.1489
Zayed, M., Abd El-Fattah, D., Abbas, R., El-Bordeny, N., Edris, S. The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics. EKB Journal Management System, 2023; 31(1): 29-40. doi: 10.21608/ajs.2022.155161.1489

The Potential Efficiency of Lactobacillus farraginis Isolated from Ruminants with a View to be Used as Animal Probiotics

Arab Universities Journal of Agricultural Sciences
Article 17, Volume 31, Issue 1, June 2023, Page 29-40  XML PDF (413.78 K)
Document Type: Original Article
DOI: 10.21608/ajs.2022.155161.1489
View on SCiNiTO View on SCiNiTO
Authors
Mona Zayedorcid 1; Dalia Abd El-Fattah email orcid 2; Rateb Abbasorcid 3; N. El-Bordeny4; Sherif Edris5
1Agricultural Microbiology Dept, Fac of Agric, Ain Shams Univ, P.O. Box 68, Hadayek Shoubra 11241, Cairo, Egypt
2Biological Agricultural Dept, Central Laboratory for Agricultural Climate, Agricultural Research Center, Dokki, Giza, Egypt
3Microbial Biotechnology Dept, GEBRI, University of Sadat City, Menofia Egypt
4Animal Production Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68 Hadayek Shoubra, 11241, Cairo, Egypt
5Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Saudi Arabia
Abstract
Probiotics refer to microorganisms that exhibit a beneficial effect on the animals’ health through intestinal microbial balance. This investigation intends to identify and define the probiotic characteristics of Lactobacillus strains isolated from the digestive systems of ruminants. Bacterial strains were isolated purified and characterized based on morphological and biochemical characteristics. The isolates were identified using the 16S rRNA gene partial sequencing method. Most of the strains exhibited a decrease in the growth by increasing the concentration of the bile salt, NaCl, increasing the temperature over 37oC, and moving toward neutrality and alkalinity of the media. Strain Lactobacillus farraginis MD_A11 revealed the lowest decrease in the growth percentage when subjected to different bile salt concentrations of 5.96, 6.61, 6.85, 7.40, 7.53, and 7.64%, NaCl% concentrations of 1.40, 5.62, 6.48, 7.36, 7.39, 7.41, 7.42, 7.28, and 13.76%, raising the temperature over 37oC being 4.19 & 3.945%, different pH levels as compared to control, and it recorded the lowest medium pH after the third day being 4.20 with titrable acidity of 0.32%. Strain Lactobacillus farraginis MD_A11 could be recommended as a probiotic feed additive for ruminants to improve their growth performance and productivity.
Keywords
16S rRNA gene sequences; Bile salt resistance; Probiotics; Ruminants; Lactobacillus farraginis
Statistics
Article View: 234
PDF Download: 125