Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease
Rashad, N., Elshamy, A., Hussien, M., Elfarargy, O., El-Helaly, A., Gomaa, A. (2022). Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease. EKB Journal Management System, 89(2), 6548-6555. doi: 10.21608/ejhm.2022.270492
Nearmeen Rashad; Abdelmonem Mohamed Elshamy; Marwa H.S. Hussien; Ola M. Elfarargy; Amira M. El-Helaly; Ahmed F. Gomaa. "Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease". EKB Journal Management System, 89, 2, 2022, 6548-6555. doi: 10.21608/ejhm.2022.270492
Rashad, N., Elshamy, A., Hussien, M., Elfarargy, O., El-Helaly, A., Gomaa, A. (2022). 'Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease', EKB Journal Management System, 89(2), pp. 6548-6555. doi: 10.21608/ejhm.2022.270492
Rashad, N., Elshamy, A., Hussien, M., Elfarargy, O., El-Helaly, A., Gomaa, A. Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease. EKB Journal Management System, 2022; 89(2): 6548-6555. doi: 10.21608/ejhm.2022.270492

Impact of Estrogen Receptor 2 Gene Polymorphisms and Expression, on Risk and Progression of Hepatocellular Carcinoma among Women with Nonalcoholic Fatty Liver Disease

The Egyptian Journal of Hospital Medicine
Article 74, Volume 89, Issue 2, October 2022, Page 6548-6555  XML PDF (821.65 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2022.270492
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Authors
Nearmeen Rashad email orcid ; Abdelmonem Mohamed Elshamy; Marwa H.S. Hussien; Ola M. Elfarargy; Amira M. El-Helaly; Ahmed F. Gomaa
vill elgamaa
Abstract
Background:Given that risk for hepatocellular carcinoma (HCC) is linked to liver cirrhosis. There is accumulating evidence that estrogen influences liver function via estrogen receptors (ERs), which are dysregulated in many cancers all over the body. Objective: We aimed to investigate the ESR2 genes (rs1256049 and rs-4986938) polymorphism and mRNA expression in patients with nonalcoholic fatty liver (NAFLD), in correlation with the risk and progression of HCC among Egyptian women with NAFLD. Patients and Methods:Case-control study enrolled fifty women with NAFLD and fifty healthy women.  Serum E2 level was determined using ELISA. Genotyping of ESR2-SNPs (rs1256049 and rs4986938) was performed by PCR-RFLP. ERβ2 mRNA values were explored by RT- PCR.
Results: There were non-significant differences concerning ERβ2 mRNA expression levels among studied groups. Concerning genotype of ESR2 (rs1256049), in NAFLD group the frequencies of the AA, GA were higher than in controls (OR = 22.62,95% CI = 2.8- 182.6, P ˂0.001 and OR = 8.7,95% CI = 1.77- 42.69, P ˂0.001 respectively). Additionally, A allele OR = 13.5,95% CI = 4.58- 39.76, P ˂0.001). There were non-significant differences in frequencies of GG, GA, and A allele between case and control groups P >0.001. Regarding ESR2 (rs1256049), the only significant parameter was A allele OR = 4.71,95% CI = 1.96-11.33, P ˂0.001 in cirrhotic NAFLD compared to non-cirrhotic NAFLD. ESR2 (rs1256049) polymorphism was significantly higher in HCC. Thus, it could be a predictor of HCC among NAFLD patients. BMI, HOMA-IR, E2, and FIB-4 scores were highly correlated with HCC in both cirrhotic and non-cirrhotic NAFLD groups by applying a logistic regression test.
Conclusion: ESR2 (rs1256049) polymorphism was significantly higher in HCC. Thus, it could be a predictor of HCC among NAFLD patients.
 
Keywords
NAFLD; HCC; Cirrhotic; ESR2 genes; Genotype
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