Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes
El-Adrosy, K., Ibrahim, M., Abdul Wahid, O., Dewedar, A. (2023). Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes. EKB Journal Management System, 4(2), 355-377. doi: 10.21608/ajbas.2022.164716.1124
Kholoud El-Adrosy; Mohsen Elsayed Ibrahim; Omar Abdul Rahman Abdul Wahid; Ahmed Dewedar. "Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes". EKB Journal Management System, 4, 2, 2023, 355-377. doi: 10.21608/ajbas.2022.164716.1124
El-Adrosy, K., Ibrahim, M., Abdul Wahid, O., Dewedar, A. (2023). 'Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes', EKB Journal Management System, 4(2), pp. 355-377. doi: 10.21608/ajbas.2022.164716.1124
El-Adrosy, K., Ibrahim, M., Abdul Wahid, O., Dewedar, A. Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes. EKB Journal Management System, 2023; 4(2): 355-377. doi: 10.21608/ajbas.2022.164716.1124

Statistical screening of process variables for improving bioethanol production using alkali-pretreated of some environmental wastes

Alfarama Journal of Basic & Applied Sciences
Article 11, Volume 4, Issue 2, April 2023, Page 355-377  XML
Document Type: Original Article
DOI: 10.21608/ajbas.2022.164716.1124
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Authors
Kholoud El-Adrosy email 1; Mohsen Elsayed Ibrahim2; Omar Abdul Rahman Abdul Wahid3; Ahmed Dewedar4
1Botany Department, Faculty of Science, Port-Said University, Port-Said, Egypt
2Department of Botany, Faculty of Science, Port Said University, Port Said, 42524, Egypt.
3Department of Botany, Faculty of Science, University of Suez Canal, Ismailia, Egypt
4Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Abstract
Bioethanol is considered the most proposed next automotive fuel generation. For the bioethanol production, the microbial conversion from some local environmental wastes (rice straw and manure) and the indigenous strains, namely Streptomyces aegyptia and Candida tropicalis were used in this study. Improving the conditions that may affect the process such as alkali pretreatment, enzymatic hydrolysis, and fermentation were investigated using the Plackett-Burman design (PBD) via submerged fermentation. X-ray diffraction (XRD) analysis revealed that the crystallinity index of pretreated rice straw (52.2%) was significantly (p-value) lower as compared to the untreated one (63.1%). The results showed that the optimization of the microbial conversion reached 6.6 folds in comparison to the unoptimized process. An improvement in enzymatic activities of Streptomyces aegyptia was achieved in the design that the maximal lignin peroxidase, Mn-dependent peroxidase, total cellulase, endocellulase, and xylanase activities were 120 U/min.l, 1020 U/min.l, 3.7 U/min.l, 57.3 U/min.l and 78.1 U/min.l, respectively. Maximum ethanol concentration and bioconversion process efficiency were 0.8 g/l and 13.3%, respectively.
Keywords
Alkali pretreatment; bioethanol; microbial conversion; Plackett-Burman design
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