CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL
M. El-Dawayati, M., I. Baker, E., H. Gomaa, A. (2007). CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL. EKB Journal Management System, 38(1), 75-95. doi: 10.21608/ajas.2007.271084
M. M. El-Dawayati; E. I. Baker; A. H. Gomaa. "CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL". EKB Journal Management System, 38, 1, 2007, 75-95. doi: 10.21608/ajas.2007.271084
M. El-Dawayati, M., I. Baker, E., H. Gomaa, A. (2007). 'CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL', EKB Journal Management System, 38(1), pp. 75-95. doi: 10.21608/ajas.2007.271084
M. El-Dawayati, M., I. Baker, E., H. Gomaa, A. CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL. EKB Journal Management System, 2007; 38(1): 75-95. doi: 10.21608/ajas.2007.271084

CRYOPRESERVATION OF IN VITRO ESTABLISHED SHOOT TIP EXPLANTS OF DATE PALM CV. ZAGHLOOL

Assiut Journal of Agricultural Sciences
Article 3, Volume 38, Issue 1, March 2007, Page 75-95  XML PDF (333.11 K)
Document Type: Original Article
DOI: 10.21608/ajas.2007.271084
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Authors
M. M. El-Dawayati1; E. I. Baker2; A. H. Gomaa3
1The Central Laboratory for Date Palm Researches and Development, Agriculture Research Center, Cairo, Egypt
2Department of Pomology, Faculty of Agriculture, Cairo University, Egypt.
3Department of Pomology, Faculty of Agriculture, Cairo University, Egyp
Abstract
Cryoprotection procedures for in vitro established shoot tip explants of Date palm cv. Zaghlool prior to cryoprservation process in liquid nitrogen were investigated in this study. This work aimed to develop simple cryoprotection procedure that can be handled easily. The first cryoprotection procedure was to study the effects of different sucrose concentrations (0.1, 0.3, 0.5, and 0.7Mol) in preculturing medium and the incubation under 5˚C or 27˚C for four weeks. The second cryoprotection procedure was to study the effects of the same previous sucrose concentrations in preculturing medium and the incubation under 27˚C for four weeks followed by desiccation procedure for two hours in an open petri dish exposed to continual current of sterile air in the laminar airflow cabinet. The third cryoprotection procedure was to study the effects of abscisic acid (ABA) concentrations (2.0, 4.0, 6.0 and 8.0 mg/L) in preculturing media and the incubation under 5˚C or 27˚C for four weeks. The fourth cryoprotection procedure was to study the effects of the same previous concentrations of (ABA) in preculturing media and incubation under 27 ˚ C for four weeks followed by the same desiccation procedure . All shoot tip explants obtained from the four cryoprotiction procedures were plunged in liquid nitrogen Dewar at (-196 oC) for one hour followed by thawing process in water bath at 40 oC. All cryopreserved shoot tip explants were cultured on recovery medium for 3 weeks to examine their potential for surviving after cryopreservation process. The results of explants viability showed that all of the tested pretreatments gave quiet results for survival. The optimal procedure that we developed was the cryoprotiction of cultured shoot tip explants on preculturing medium supplemented with 0.5 M of sucrose concentration for four weeks and incubated at 27oC followed by air desiccation treatment prior to cryopreservation process. The cryopreserved explants from this cryoprotiction procedure revealed high survival and could resume their life cycle in normal manner on the recovery medium. Date palm explants cryopreservation needs more studies to achieve better results in germplasm conservation
Keywords
cryopreservation; in vitro; shoot tip; date palm
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