Green Bio Estimation of Co-Administered Non-Steroidal Anti Inflammatory Drug Diclofenac and Leflunomide in Rat Plasma by New HPLC Method | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 65, Issue 132, December 2022, Page 1307-1316 PDF (579.94 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.156644.6786 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ossama Abdullatef  1; Yasmine Khalifa1; Samir Morshedy 2; Wael Talaat2
| ||||
| 1Faculty of Pharmacy, Pharmaceutical Chemistry Department, Pharos University, Smouha, Alexandria, Egypt | ||||
| 2Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Damnhour University, Damnhour, Egypt | ||||
| Abstract | ||||
| An eco-friendly HPLC- DAD method is introduced for the bioanalysis of co-administered non-steroidal anti-inflammatory drug diclofenac and leflunomide in rat plasma. Leflunomide and NSAIDs are commonly prescribed in combination This method aimed to estimate the co-adminstered leflunomide and diclofenac combination for their simultaneous separation and quantification The method was conducted with gradient elution of a mobile phase composed of ethanol and phosphate buffer with a flow rate of 1 mL/min over a Zorbax Eclipse Plus-C18 (4.6 × 250 mm × 5 µm) column. The drug peaks were picked using a diode array detector at 275 nm. The developed method was validated according to ICH guidelines in terms of accuracy, precision, linearity, range, LOD and LOQ. The concentration range was found to be linear in the range of 2 – 60 µg/mL. The LOD and LOQ values were found to be very small (0.16, 0.47 μg/mL and 0.22, 0.68 μg/mL) for Diclofenac and Leflunommide respectively. The % RSD and the % R were found within the acceptable range. The method was applied for in vitro and in vivo analysis of diclofenac and leflunomide with mean recoveries of 97.5 to 102.30 and 97.31 to 101.20 respectively. | ||||
| Keywords | ||||
| Green method; Bioanalysis; NSAID; Leflunomide | ||||
|
Statistics Article View: 160 PDF Download: 110 |
||||
