Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep
Ezzat, M., Abdelhamid, M., ELgedway, A., EL-Tarabili, R., Arnaout, M. (2022). Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep. EKB Journal Management System, 27(2), 223-233. doi: 10.21608/scvmj.2022.271372
Mahmoud Ezzat; Marwa Abdelhamid; Attia ELgedway; Reham EL-Tarabili; Marwa Arnaout. "Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep". EKB Journal Management System, 27, 2, 2022, 223-233. doi: 10.21608/scvmj.2022.271372
Ezzat, M., Abdelhamid, M., ELgedway, A., EL-Tarabili, R., Arnaout, M. (2022). 'Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep', EKB Journal Management System, 27(2), pp. 223-233. doi: 10.21608/scvmj.2022.271372
Ezzat, M., Abdelhamid, M., ELgedway, A., EL-Tarabili, R., Arnaout, M. Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep. EKB Journal Management System, 2022; 27(2): 223-233. doi: 10.21608/scvmj.2022.271372

Prevalence Phenotypic Characterization of Listeria Monocytogenes Isolated from Diseased Sheep

Suez Canal Veterinary Medical Journal. SCVMJ
Article 3, Volume 27, Issue 2, December 2022, Page 223-233  XML PDF (632.57 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2022.271372
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Authors
Mahmoud Ezzatorcid 1; Marwa Abdelhamid2; Attia ELgedway3; Reham EL-Tarabili* 1; Marwa Arnaout4
1Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt
2Microbiology Department, Faculty of Veterinary Medicine, Zagazig University
3Microbiology Department, Animal Health Research Institute, Dokki, Egypt
4Veterinary quarantine international Cairo Airport
Abstract
Listeriosis is the third most common food-borne illness and is considered one of the most dangerous bacterial zoonotic infections globally. This research aimed to isolate and characterize Listeria monocytogenes (L. monocytogenes) from diseased sheep in Egypt. A total of 240 samples collected aseptically from sheep were subjected to isolation and biochemical characterization of L. monocytogenes. L. monocytogenes are Gram-positive rods that are both aerobic and facultatively anaerobic. Thirty-one listeria isolates (12.9%) were recovered from diseased sheep. All tested isolates were positive for catalase, Voges-Proskauer, carbohydrate fermentation, esculin, gelatin hydrolysis, and methyl red tests. Meanwhile, they were negative for oxidase, indole, urease, and nitrate reduction tests and did not utilize citrate in the citrate utilization test. Listeria isolates produced yellow butt/yellow slant on the TSI agar medium with no H2S or gas production. Therefore, this work focused on the isolation of L. monocytgenes from diseased sheep and biochemical characterization of the recovered isolates using traditional and Microbact™ 12L listeria identification systems.
Keywords
Listeria monocytogenes; Sheep; Phenotypic Identification; Biochemical Tests
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