Effect of some Abiotic Factors on Growth, Glycerol and β-carotene Accumulation by Dunaliella bardawil | ||||
| Egyptian Journal of Botany | ||||
| Article 1, Volume 56, Issue 3, September 2016, Page 559-571 PDF (518.3 K) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2016.2718 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| UNALIELLA bardawil can produce and accumulate large amounts of glycerol and β-carotene in response to stress conditions. The effect of pH, salinity, light intensity and algal inocula as stress factors on growth, β-carotene and glycerol production in D. bardawil was assessed in the present study. The growth profile showed linear relationship for chlorophyll a content, optical density measurementsand cell count with time up to the end of the experiment (42 days). The mean growth rate, relative growth rate and number of recycling reached their maximum values after 4 days of growth while generation time recorded the least value at the same time. D. bardawil can grow in wide pH range (5.5-9.5) with maximum growth (chlorophyll a and cell count) and glycerol content at pH 7.5 whereas the best pH value for β-carotene production was 8.5. Increasing algal inoculum caused significant increase in growth and β-carotene content while non significant increase in glycerol was observed. Regarding salinity stress, 4 M NaCl was the best concentration for glycerol production and 2.5 M NaCl for growth and β-carotene accumulation. High light stress (292.5 μE m-2 s-1) enhanced β-carotene production and the ratio of β-carotene to chlorophyll a reached 1.59. | ||||
| Keywords | ||||
| Dunaliella bardawil; Β-CAROTENE; glycerol; Stress factors; salinity; Light intensity; pH; algal inoculum | ||||
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