Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities
Mostafa, S., Abdou, R., El-Tanbouly, N., El-sayed, A. (2023). Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities. EKB Journal Management System, 66(8), 167-176. doi: 10.21608/ejchem.2022.167475.7065
Sherifa Fahmy Mostafa; Rabab Mahrous Abdou; Nebal Darweesh El-Tanbouly; Aly Mohamed El-sayed. "Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities". EKB Journal Management System, 66, 8, 2023, 167-176. doi: 10.21608/ejchem.2022.167475.7065
Mostafa, S., Abdou, R., El-Tanbouly, N., El-sayed, A. (2023). 'Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities', EKB Journal Management System, 66(8), pp. 167-176. doi: 10.21608/ejchem.2022.167475.7065
Mostafa, S., Abdou, R., El-Tanbouly, N., El-sayed, A. Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities. EKB Journal Management System, 2023; 66(8): 167-176. doi: 10.21608/ejchem.2022.167475.7065

Metabolites Profiling of Pouteria Campechiana (Kunth) Baehni Different Organs Using UPLC-PDA-MS and its Biological Activities

Egyptian Journal of Chemistry
Volume 66, Issue 8, August 2023, Page 167-176  XML PDF (689.68 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.167475.7065
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Authors
Sherifa Fahmy Mostafa1; Rabab Mahrous Abdou email orcid 2; Nebal Darweesh El-Tanbouly2; Aly Mohamed El-sayed2
1pharmacognosy department, Faculty of Pharmacy, Cairo Univversity, Cairo, Egypt
2Pharmacognosy department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
Abstract
Pouteria campechiana (Kunth) Baehni is native to America and is cultivated in Egypt for its edible fruit. UPLC-PDA-qTOF-MS technique was performed to detect the metabolites profile of three organs of the Egyptian plant, namely, leaves, seeds and pericarps. UPLC-MS spectra was analyzed using Principal Component Analysis to explore the relative variability within organs. The antioxidant and antimicrobial activities of the ethanol extracts were assessed by free radical 1,1-diphenyl-2-picrylhydrazyl scavenging activity and agar well diffusion assay methods, respectively. The IC50 values of the leaves, seeds and pericarps ethanol extracts were 73.68±8.12, 235.615±10.3 and 858.952±18.16 μg/mL, respectively. They showed antimicrobial activity against three bacterial and three fungal strains out of the eight tested microorganisms. Sixty-three chromatographic peaks were detected among organs. Two main principle components (PC1 and PC2) were responsible for the discrimination of the three organs. Flavonol glycosides were found highest in the leaves. Taxifolin and ursolic acid were predominant in the seeds. Unsaturated fatty acids contributed the most in the discrimination of pericarps from leaves and seeds.
Keywords
Pouteria campechiana; UPLC-MS; antioxidant; antimicrobial; principle component analysis
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