Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.
Aly, S., Saad, M., Hendam, A., Abdel-Hamied, A., Al-Sadek, A., Madboly, E., Barakat, H., El-Atroush, H. (2022). Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.. EKB Journal Management System, 14(2), 311-330. doi: 10.21608/eajbsc.2022.272232
Sara Aly; Mohamed E. Saad; Ashraf Hendam; Amina Abdel-Hamied; Ahmed Farouk Al-Sadek; E. A. Madboly; Hoda S. Barakat; H. El-Atroush. "Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.". EKB Journal Management System, 14, 2, 2022, 311-330. doi: 10.21608/eajbsc.2022.272232
Aly, S., Saad, M., Hendam, A., Abdel-Hamied, A., Al-Sadek, A., Madboly, E., Barakat, H., El-Atroush, H. (2022). 'Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.', EKB Journal Management System, 14(2), pp. 311-330. doi: 10.21608/eajbsc.2022.272232
Aly, S., Saad, M., Hendam, A., Abdel-Hamied, A., Al-Sadek, A., Madboly, E., Barakat, H., El-Atroush, H. Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.. EKB Journal Management System, 2022; 14(2): 311-330. doi: 10.21608/eajbsc.2022.272232

Whole Genome Sequencing of Date Palm (Phoenix dactylifera L.) Cultivars Using NGS.

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Volume 14, Issue 2, December 2022, Page 311-330  XML PDF (1.24 MB)
Document Type: Original Article
DOI: 10.21608/eajbsc.2022.272232
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Authors
Sara Aly1; Mohamed E. Saad2; Ashraf Hendam3; Amina Abdel-Hamied4; Ahmed Farouk Al-Sadek3; E. A. Madboly5; Hoda S. Barakat1; H. El-Atroush1
1Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt.
2Department of Nucleic acid, proteomics and Genomics, Genomics facility central lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center ARC, Egypt. - Department of Biology, Faculty of Science, Taibah University, Almadinah Almonawarah, Kingdom of Saudi Arabia.
3Bioinformatics Unit, Central Lab for Agricultural Experts Systems, Agricultural Research Center (ARC), Giza, Egypt.
4Department of Nucleic acid, proteomics and Genomics, Genomics facility central lab, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center ARC, Egypt.
5The Central Laboratory for Date Palm Research and Development (CLDPRD), Agricultural Research Center (ARC), Giza, Egypt.
Abstract
Date palm (Phoenix dactylifera L.) is related to the family Arecaceae, which is considered one of the most ancient economically cultivated crops. Mainly, it is grown in the arid regions of the Middle East and North Africa.  The crucial matter in maintaining the diverse number of date palm cultivars in Egypt is its biodiversity conservation of it. In order to progress programs and cultivar characterization and conservation to combat genetic erosion, we must estimate the genetic variability and right date palm cultivar identification this is the important point to present a comprehensive investigation for Egyptian date palm genome variations and develop novel DNA markers (SNPs and indels) in four date palm cultivars using SOLiD sequencing.
Keywords
Whole Genome (WGS) Sequencing -Next Generation Sequencing (NGS); SNP; Indel; DNA Markrs; Date Palm
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