Effect of Bone Marrow-Derived Mesenchymal Stem Cells versus Probiotics in the Treatment of experimentally induced Acute Colitis in Rats: A Histological and an Immunohistochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 04 December 2022 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.170365.1795 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Doaa Ramadan sadek   1; Rania Hamdy Elsyade 2
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| 1Lecturer in Histology and Cell Biology Department, Faculty of Medicine, Ain Shams University. Cairo, Egypt | ||||
| 2Anatomy and embryology, faculty of Medicine, Helwan university, Cairo, Egypt. | ||||
| Abstract | ||||
| Background: Inflammatory bowel disease (IBD) is a progressive inflammatory condition of the gastrointestinal tract (GIT). Mesenchymal stem cells have immunomodulatory capabilities and probiotics may modify the pathophysiology of gut disorders and have positive effects on the gut microbiota AIM: Compare the effect of BM-MSCs versus probiotics on the treatment of acute colitis in adult male albino rats. METHODS: Forty adult male albino rats weighed (200-220 gm) were equally divided into five groups. Group I (control). Group II (Acute colitis). Group III (recovery): sacrificed 14 days from induction of acute colitis (IAC). Group IV (Colitis + BM-MSCs): received a single injection of 1ml of PBS containing 1×106 BM-MSCs. Group V (colitis + Probiotic): received 1x109 CFU/ml/100 gm of b.w./day of Lactobacillus Plantarum orally. In groups IV and V, rats were treated three days after IAC and sacrificed 14 days after this induction. RESULTS: The acute colitis group showed mucosal ulceration, cellular infiltration, and decreased number of goblet cells. The area percentage of collagen and TNF-α immune stain revealed a significant increase while the area percentage of PCNA-positive nuclei immune stain significantly decreased. The recovery group showed incomplete recovery of these structural changes. The histological architecture of the colon was restored following the treatment with stem cells and probiotics. CONCLUSION: Treatment with stem cells or probiotics restored the integrity of the colonic mucosa in a rat model of acute colitis. Probiotics therapy was superior to stem cell therapy as evidenced by allowing rapid division and differentiation of stem cells into goblet cells that form mucous barriers. | ||||
| Keywords | ||||
| BM-MSCs; Probiotic; Acute Colitis; PCNA; TNF- α | ||||
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