Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies
Alkhudher, T., Zaky, M., Metwally, L., El Kazzaz, W. (2023). Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies. EKB Journal Management System, 4(2), 274-289. doi: 10.21608/ajbas.2022.161965.1120
Tahany K. Alkhudher; Mahmoud Mohamed Mohamed Zaky; Lobna Metwally; Waleed M. El Kazzaz. "Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies". EKB Journal Management System, 4, 2, 2023, 274-289. doi: 10.21608/ajbas.2022.161965.1120
Alkhudher, T., Zaky, M., Metwally, L., El Kazzaz, W. (2023). 'Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies', EKB Journal Management System, 4(2), pp. 274-289. doi: 10.21608/ajbas.2022.161965.1120
Alkhudher, T., Zaky, M., Metwally, L., El Kazzaz, W. Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies. EKB Journal Management System, 2023; 4(2): 274-289. doi: 10.21608/ajbas.2022.161965.1120

Incidence, Antibiogram and MAR Index of E. coli from Wastewater Treatment Plant, Reclaimed Forest Soil and Affected Water Bodies

Alfarama Journal of Basic & Applied Sciences
Article 6, Volume 4, Issue 2, April 2023, Page 274-289  XML
Document Type: Original Article
DOI: 10.21608/ajbas.2022.161965.1120
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Authors
Tahany K. Alkhudher1; Mahmoud Mohamed Mohamed Zaky2; Lobna Metwally3; Waleed M. El Kazzaz email 4
1Botany Department, Faculty of Science, Port Said University, Port Said, Egypt
2Department of Botany, Faculty of Science, Port Said University, Port Said, 42524, Egypt.
3Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
4Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract
Wastewater is a major collector for antibiotic resistant and pathogenic microorganisms posing high risk on public health and environmental. Even efficient secondary sewage treatment does not ensure appropriate removal of pathogens especially MDR E. coli. This study aimed to shed light on the prevalence and antimicrobial resistance of E. coli in raw wastewater, treated water, sludge, and forest soil irrigated with treated water from the Serapium wastewater treatment plant in Ismailia, Egypt. Samples were collected monthly during the period February 2018 to January 2019 from different wastewater treatment stages as well as sludge, reclaimed soil and affected water sources. E. coli isolated on HiChrome E. coli agar and verified by indole reagent system. Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing for 19 different antibiotics. E. coli mean count ranged between 4.7×104 and 6.3×105 MPN/100mL in the influent and 2.1×102 and 6.3×103 MPN/100mL for effluent indicating high bacterial load in the effluent. Antimicrobial susceptibility rate for the selected 337 E. coli strains was highest for ampicillin (29.2%), tetracycline (22.5%), and ciprofloxacin (16.3%) while lower resistance for ertapenem (2.4%), imepenim (2.3%), and meropenim (2.3%,) and 4.4% azithromycin. ESBL producing E. coli represents 20.67% of the isolates. Notwithstanding, upwards of 103 MPN/100mL E. coli with high MAR index (>0.2) has reached the receiving ecosystem and thus the processes of sewage treatment contribute to the spread of antibiotic resistant bacteria into the environment.
Keywords
MAR index; MDR E. coli; Reclaimed soil; Wastewater treatment
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