The protective role of l-carnitine against cisplatin induced testicular toxicity in the adult albino rat | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 05 December 2022 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.172815.1803 | ||||
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| Authors | ||||
Sultan H. Ali  1; Rania H. Elsyade 2; Kariman H. Abdel Wahab3; Manal H. AL Badawi 4
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| 1Human Anatomy and Embryology, Faculty of medicine, Helwan University, Cairo, Egypt. | ||||
| 2Lecturer of Anatomy, Helwan faculty of medicine | ||||
| 3Department of pathology, Faculty of medicine, Helwan University, Cairo, Egypt. | ||||
| 4faculty of medicine Helan univercity | ||||
| Abstract | ||||
| Background: Testicular damage is one of the most deleterious effects whenever cisplatin (CP) is employed in cancer treatment. CP results in male infertility. L-carnitine (LC) is vitamin like substance with an antioxidant activity. L-carnitine improves spermatogenesis, sperm maturation and motility AIM of the work: Assess the protective role of LC in cisplatin-induced testicular toxicity. Material and methods: Forty adult male albino rats (200-250 gram each) were divided to four groups. The first group: Control. The group II: LC was intraperitoneal (IP) injected (500 mg/kg/d) for fifteen days. Group III: CP was injected with a only one dose of 7 mg/kg CP (IP) at 6th day. Group IV: LC plus CP received treatment of both groups II and III. Semen analysis was performed for estimating sperm count and motility. Testicular sections were examined under light microscopy using H&E, PAS and anti-inducible nitric oxide synthase (iNOS) stainings. Transmission electron microscope was also done. Results: Cisplatin caused significant decline in the body weight, absolute and relative testicular weight, sperm count, sperm motility, seminiferous tubular diameter and the germinal lining height in relation with other groups, with a significant improvement in the CP plus LC group. Specimens from CP treated animals showed a thick and destructed seminiferous tubules (ST) basal lamina, obvious disorganized and atrophied ST, spermatogenic arrest, atrophied leydig cells. LC improves these changes with variable preservation of spermatogenic cells. Inducible NOS staining in the CP treated group showed a moderate positive reaction, other groups showed a negative or weak reaction. Transmission electron photomicrography of the CP group showed large cytoplasmic vacuoles, small distorted mitochondria and large lipid droplets in most spermatogonic cells; abnormally shaped spermatozoa. Conclusion: L-carnitine partially attenuates CP related histological changes of the testicular tissue and improves the quality parameters of the sperm, ensuring the protective effect of LC. | ||||
| Keywords | ||||
| Cisplatin; L-carnitine; Testicular toxicity; iNOS | ||||
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