Silver Nanoparticles Display Inhibitory Effect against Drug-Resistant Pathogenic Candida Isolates from Different Clinical Specimens | ||||
| Egyptian Journal of Botany | ||||
| Article 20, Volume 63, Issue 1, January 2023, Page 305-314 PDF (1.12 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2022.155982.2101 | ||||
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| Authors | ||||
Rasha M. Elnagar   1; Mohammed Elshaer2; Mona A. Abd El-Raouf1
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| 1Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||||
| 2Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||||
| Abstract | ||||
| The resistance of Candida to antifungal agents is an emerging global health problem, especially with the emergence of new species. Therefore, the focus has shifted to alternative agents like silver nanoparticles (AgNPs), received significant attention. The aim of this study was to evaluate the in vitro antifungal susceptibility of AgNPs against resistant Candida isolates from various clinical specimens. The antifungal effect of AgNPs was assessed by the broth microdilution method using different concentrations (0.062-1 µg/mL). The Minimum Inhibitory Concentration (MIC50) and Minimum Fungicidal Concentration (MFC) of AgNPs were determined. As a result, of 109 recovered Candida isolates, 65.1%, and 34.9% were C. albicans andnon-albicans Candida (NAC), respectively. Moreover, 35.8% of the Candida isolates were non-susceptible to conventional antifungal drugs. The MIC50 of AgNPs against resistant isolates was lower (0.125-1 µg/mL) than that of fluconazole (1-64 µg/mL), itraconazole (0.016-4 µg/mL), voriconazole (0.016-4 µg/mL), and amphotericin B (0.016-1 µg/mL). The scanning electron micrograph revealed that AgNPs treatment alteredthe cell morphology of Candida. In conclusion, AgNPs exhibited a notable fungicidal effect against resistant Candida isolates and may be an adequate substitute for antifungal agents. | ||||
| Keywords | ||||
| Antifungal agents; Candida spp; Resistance; Silver nanoparticles (AgNPs) | ||||
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