Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients
karras, M., El Mashad, N., Refaie, A., Mofreh, M., Salah, H., Zaki, M. (2022). Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients. EKB Journal Management System, 89(2), 7103-7111. doi: 10.21608/ejhm.2022.273051
Mary Samy karras; Noha El Mashad; Ayman Refaie; Mohamed Mofreh; Hazem H. Salah; Mayssa Elsayeed Zaki. "Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients". EKB Journal Management System, 89, 2, 2022, 7103-7111. doi: 10.21608/ejhm.2022.273051
karras, M., El Mashad, N., Refaie, A., Mofreh, M., Salah, H., Zaki, M. (2022). 'Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients', EKB Journal Management System, 89(2), pp. 7103-7111. doi: 10.21608/ejhm.2022.273051
karras, M., El Mashad, N., Refaie, A., Mofreh, M., Salah, H., Zaki, M. Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients. EKB Journal Management System, 2022; 89(2): 7103-7111. doi: 10.21608/ejhm.2022.273051

Comparison between Serological and Molecular Diagnosis of Epstein Barr Virus Infection among Egyptian Renal Transplant Recipients

The Egyptian Journal of Hospital Medicine
Article 150, Volume 89, Issue 2, October 2022, Page 7103-7111  XML PDF (729.86 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2022.273051
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Authors
Mary Samy karras email orcid ; Noha El Mashad; Ayman Refaie; Mohamed Mofreh; Hazem H. Salah; Mayssa Elsayeed Zaki
Urology and Nephrology center, Mansoura University
Abstract
Background: Kidney transplantation is the best treatment for end-stage kidney diseases. Lifelong immunosuppression preserves graft function. However, they usually lead to severe viral infections; as Epstein Barr Virus (EBV) which lead to development Post Transplant Lymphoproliferative Disorders (PTLD). Objectives: The aim of this study is to compare between serological and molecular assays as methods of detection of EBV infection. Presumed risk factors for development of EBV infection and its impact on transplant outcome were studied. Patients and Methods: A total of 50 Egyptian kidney transplant recipients received their renal allografts from living-related donors were studied. Recipients were tested for EBV infection by serological markers; anti-EBV viral capsid antigen (VCA) IgM and molecular assay by detection BamHI region by PCR. Different co-morbid risk factors for development of EBV infection (pre-transplant hemodialysis & blood transfusion, diabetes mellitus, CMV, and HCV infection) were studied. Likewise, the impact of EBV infection on transplant outcomes was evaluated. Results: Of the 50 patients, 66% were positive for VCA IgM and 42% were positive BamHI region of EBV. BamHI positivity was significantly correlated with duration of transplantation, and severity of rejections episodes. On the other hand, none of these risk factors were correlated with the positivity of VCA IgM. Among the studied recipients, EBV infection detected by either serological or molecular assay has no impact on the transplant outcome.
Conclusions: Althoughserological diagnosis for EBV infection is a simple method for screening and follow-up. Yet, molecular diagnosis seems to be more accurate diagnostic test.
 
Keywords
Epstein Barr Virus; Kidney transplantation; Serological Diagnosis; Molecular Diagnosis; Case series; Mansoura University
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