Evaluation of the Potential role of MicroRNA 126 in Hepatitis C infection patients treatment Outcomes | ||||
| Benha Veterinary Medical Journal | ||||
| Volume 42, Issue 2, September 2022, Page 19-24 PDF (1.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2022.147218.1538 | ||||
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| Authors | ||||
Dalia Mohamed Shaker Elkholy   1; Mohamed Mahfouz2
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| 11Department of Biochemistry & Faculty of Veterinary Medicine, Benha University | ||||
| 2Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt | ||||
| Abstract | ||||
| Background: Hepatitis C virus (HCV) infection is a worldwide problem that is likely the cause of chronic hepatitis, liver cirrhosis, liver failure, and may underlie the development of hepatocellular carcinoma (HCC). Objectives: The ability of plasma level of MiR-126 gene expression determination to differentiate patients with HCV infection according to the probable response to treatment was evaluated. Patients and methods: Blood samples of 43 patients and 17 controls were obtained and processed for determination of serum aspartate transaminase (AST), alanine transaminase (ALT) and alpha-fetoprotein (AFP) in addition to plasma level of MiR-126 gene expression.The percentage of change in post-treatment sample was calculated. Results: Treatment significantly reduced serum AFP and plasma MiR-126 expression levels, while serum AST and ALT were non-significantly reduced. The percentage of change in post-treatment serum AFP and plasma MiR-126 were positively correlated. Receiver operating characteristic (ROC) curve analysis defined high pre-treatment serum ALT and low pre-treatment plasma MiR-126 levels as sensitive and specific predictors for response to treatment, respectively with moderate accuracy. Multivariate Regression analysis defined the low pre-treatment gene expression level of MiR-126 as the significant predictor for response to treatment. Conclusion: The gene expression of MiR-126 is correlated with the results of laboratory diagnostic tests for HCV infection, but has a significantly higher diagnostic value. Low gene expression of MiR-126 can discriminate samples of HCV responders to treatment. | ||||
| Keywords | ||||
| Hepatitis C virus; MicroRNA-126; liver enzymes; Alpha-fetoprotein | ||||
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